首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前动力蛋白2(PROK2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PROK2含量。PROK2即促动素2，是分泌性蛋白。其作为重要靶点，在人体生理活动如血管生成、神经发育等方面发挥作用。研究机制方面，它主要通过与特定受体结合，激活下游信号通路，进而影响细胞行为，目前在肿瘤、神经系统疾病等研究中受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品可为科研人员提供可靠工具，用于探索PROK2在生殖生物学、肿瘤发生机制或神经内分泌调控中的功能，亦可用于评估细胞模型、动物组织或生物体液中的蛋白表达水平变化，为相关疾病的分子机制研究及药物开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BV8 ELISA Kit; Bv8 homolog ELISA Kit; MIT1 ELISA Kit; PK2 ELISA Kit; PROK2 ELISA Kit; PROK2_HUMAN ELISA Kit; Prokineticin-2 ELISA Kit; Protein Bv8 homolog ELISA Kit
缩写：PROK2
Uniprot No.：Q9HC23
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PROK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 86-91 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 85-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human PROK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.3452.2452.2952.1602001.7641.6641.7141.5791001.1121.1021.1070.972500.7130.6930.7030.568250.4220.4120.4170.28212.50.3050.2950.3000.1656.250.2120.2020.2070.07200.1350.1340.135 | ||||||
货期：3-5 working days
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