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产品描述：人前动力蛋白1(PROK1/EG-VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清样本中的PROK1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探索PROK1在生殖生物学、内分泌调控及血管生成相关疾病中的分子机制；适用于基础科研中对血清样本的批量检测，如生殖系统疾病模型研究、肿瘤微环境分析或激素调控相关实验，为探索PROK1在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Black mamba toxin related protein ELISA Kit; EG VEGF ELISA Kit; EG-VEGF ELISA Kit; EGVEGF ELISA Kit; Endocrine-gland-derived vascular endothelial growth factor ELISA Kit; Mambakine ELISA Kit; PK1 ELISA Kit; PRK1 ELISA Kit; PROK1 ELISA Kit; PROK1_HUMAN ELISA Kit; Prokineticin 1 ELISA Kit; Prokineticin-1 ELISA Kit
缩写：PROK1
Uniprot No.：P58294
种属：Homo sapiens (Human)
样本类型：serum
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EG-VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 100-108 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 92-99 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 85-92 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 96-103 | ||||||
回收率：
| The recovery of human EG-VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 100-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.6252.7412.6832.558102.2682.1662.2172.09251.6351.5361.5861.4612.51.0531.0441.0490.9241.250.7110.6880.7000.5750.6250.4300.4360.4330.3080.3120.2820.2770.2800.15500.1210.1290.125 | |||||||
货期：3-5 working days
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