| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PML; MYL; RNF71; TRIM19; Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19 |
| Entrez GeneID | 5371 |
| WB Predicted band size | Calculated MW: 98 kDa; Observed MW: 98,117 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser518 of human PML Protein |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇涉及Phospho-PML (Ser518)抗体的参考文献及其摘要内容：
---
1. **"DNA damage-induced phosphorylation of PML mediates its interaction with the SUMO E3 ligase PIAS1 to regulate apoptosis"**
*Author: Zhong S et al.*
**摘要**：研究报道DNA损伤通过ATM激酶磷酸化PML蛋白(Ser518位点)，促进其与PIAS1的结合，进而调控SUMO化修饰和细胞凋亡。该文献使用Phospho-PML (Ser518)抗体验证DNA损伤后PML的磷酸化依赖性功能。
2. **"PML nuclear bodies: dynamic sensors of DNA damage and cellular stress"**
*Author: Dellaire G, Bazett-Jones DP*
**摘要**：综述PML核小体在DNA损伤应答中的作用，指出Ser518磷酸化是PML响应氧化应激和DNA断裂的关键事件。文中引用利用该抗体的实验数据，证明PML磷酸化促进核小体重组和修复因子招募。
3. **"Post-translational modification of PML regulates cell fate decisions in response to DNA damage"**
*Author: Salomoni P et al.*
**摘要**：研究发现PML的Ser518位点磷酸化由AKT激酶介导，抑制其促凋亡功能并促进细胞存活。该抗体被用于Western blot和免疫荧光实验，揭示PML磷酸化在肿瘤发生中的潜在机制。
---
以上文献均通过Phospho-PML (Ser518)抗体探讨了该修饰在DNA损伤应答、细胞命运调控中的分子机制。如需具体DOI或期刊信息，可进一步补充关键词检索。
The Phospho-PML (Ser518) antibody is a specialized tool used to detect the phosphorylated form of the Promyelocytic Leukemia (PML) protein at serine residue 518. PML is a key component of PML nuclear bodies (PML-NBs), dynamic subnuclear structures involved in diverse cellular processes, including transcriptional regulation, apoptosis, DNA damage response, and antiviral defense. Phosphorylation at Ser518 is a critical post-translational modification that modulates PML's functional interactions and structural organization within PML-NBs. This modification is often induced by cellular stress, DNA damage, or specific kinase signaling pathways (e.g., CK2. ATM/ATR), influencing PML's role in tumor suppression, cell cycle control, and antiviral responses.
The antibody is widely used in research to study PML's involvement in diseases such as acute promyelocytic leukemia (APL), where PML fusion proteins disrupt normal nuclear body function, as well as viral infections (e.g., herpesviruses) that target PML-NBs for immune evasion. By specifically recognizing the phosphorylated Ser518 epitope, this antibody helps elucidate dynamic changes in PML activity under pathological or experimental conditions, aiding in the understanding of PML-NB regulation, stress signaling, and therapeutic targeting. Its applications span Western blotting, immunofluorescence, and immunohistochemistry, providing insights into PML's context-dependent roles in health and disease.
×
