| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein NDRG1; Differentiation-related gene 1 protein; DRG-1; RTP; Rit42; NDRG1; CAP43; DRG1; RTP; targ1; TDD5; tdds |
| Entrez GeneID | 10397 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser330 of human NDRG1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-NDRG1 (Ser330)抗体的3篇参考文献，按研究背景和抗体应用场景分类：
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1. **文献名称**：*NDRG1 phosphorylation by SGK1 regulates cellular stress response and promotes oncogenic signaling*
**作者**：Murray JT et al.
**摘要**：研究揭示SGK1激酶在氧化应激条件下磷酸化NDRG1的Ser330位点，使用Phospho-NDRG1 (Ser330)抗体通过Western blot验证。发现该修饰通过抑制HIF-1α降解促进肿瘤细胞存活，为靶向应激信号通路提供依据。
2. **文献名称**：*Phosphorylation of NDRG1 by Akt is required for hypoxia-induced autophagy*
**作者**：Chen Z et al.
**摘要**：通过Phospho-NDRG1 (Ser330)特异性抗体，证实低氧激活的Akt直接磷酸化NDRG1的Ser330.触发自噬并增强乳腺癌细胞耐药性。研究强调该位点磷酸化在肿瘤代谢适应中的关键作用。
3. **文献名称**：*Antibody-based detection of NDRG1 phosphorylation as a biomarker for metastatic prostate cancer*
**作者**：Liu R et al.
**摘要**：开发基于Phospho-NDRG1 (Ser330)抗体的免疫组化方法，证明前列腺癌组织中Ser330磷酸化水平与转移风险正相关，提示其作为预后生物标志物的潜力。
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**备注**：上述文献为模拟示例，实际引用需通过PubMed/Google Scholar检索关键词如“Phospho-NDRG1 Ser330”或抗体货号(如CST #5484)，并优先选择抗体公司官网提供的应用文献。
The Phospho-NDRG1 (Ser330) antibody detects N-myc downstream-regulated gene 1 (NDRG1) when phosphorylated at serine 330. a post-translational modification linked to its functional regulation. NDRG1 is a stress-responsive protein involved in cell differentiation, growth arrest, and tumor suppression. Its activity is modulated by phosphorylation through various kinases, including serum/glucocorticoid-regulated kinase 1 (SGK1) and protein kinase C (PKC). Phosphorylation at Ser330 is associated with cellular responses to hypoxia, DNA damage, or hormonal signaling, such as androgen receptor pathways in prostate cancer. This modification may influence NDRG1’s role in inhibiting metastasis, regulating metal ion homeostasis, or mediating stress adaptation.
The antibody is widely used in cancer research, particularly in studies exploring NDRG1’s tumor-suppressive functions in prostate, breast, and colorectal cancers. It helps assess phosphorylation status via techniques like Western blotting, immunofluorescence, or immunohistochemistry, providing insights into pathway activation or disease progression. Dysregulated NDRG1 phosphorylation has been implicated in chemoresistance, poor prognosis, and metastatic behavior, making this antibody a tool for studying biomarkers or therapeutic targets. Specificity for the phosphorylated Ser330 epitope ensures accurate detection of active NDRG1 conformations, aiding mechanistic studies in both physiological and pathological contexts.
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