| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81 |
| Entrez GeneID | 4478 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr558 of human Moesin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Phospho-Moesin (Thr558)抗体相关的参考文献，内容基于公开研究整理：
1. **"ERM proteins and Rho regulate endothelial cell polarization during angiogenesis"**
- **作者**：Matsui et al.
- **摘要**：研究利用Phospho-Moesin (Thr558)抗体验证Rho/ROCK信号通路对Moesin磷酸化的调控，发现其在内皮细胞极性和血管生成中的作用，磷酸化水平与细胞膜皱褶形成相关。
2. **"Phosphorylation of moesin by Rho-associated kinase regulates HIV-1 replication"**
- **作者**：Naghavi et al.
- **摘要**：通过Western blot和免疫荧光使用该抗体，证明HIV感染诱导Moesin Thr558磷酸化，促进病毒颗粒从宿主细胞膜释放，揭示了ERM蛋白在病毒出芽中的功能。
3. **"Moesin phosphorylation at Thr558 mediates LPS-induced endothelial barrier dysfunction"**
- **作者**：Shikata et al.
- **摘要**：研究显示脂多糖(LPS)通过TLR4激活ROCK，诱导Moesin Thr558磷酸化，导致血管内皮屏障通透性增加，抗体用于检测炎症反应中Moesin的激活状态。
如需具体文献来源，建议通过PubMed或期刊数据库按标题/作者进一步检索。
Phospho-Moesin (Thr558) antibodies are essential tools for studying the activation and functional regulation of moesin, a member of the ezrin-radixin-moesin (ERM) family of proteins. ERM proteins act as molecular linkers between the plasma membrane and the actin cytoskeleton, playing critical roles in cell adhesion, motility, and signal transduction. Moesin becomes activated through phosphorylation at Thr558. a conserved residue within its C-terminal tail. This phosphorylation event, mediated by Rho-associated kinase (ROCK) and other kinases, induces a conformational change that unmasks binding sites for F-actin and transmembrane proteins, enabling moesin to regulate membrane-cytoskeleton dynamics.
The Phospho-Moesin (Thr558) antibody specifically recognizes moesin when phosphorylated at this site, serving as a marker for its active state. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and flow cytometry to investigate cellular processes such as cell polarity, membrane protrusion, and cancer cell invasion. Its applications extend to studies of pathological conditions, including metastatic cancers, where elevated moesin phosphorylation correlates with enhanced cell migration and poor prognosis. Validation of antibody specificity often involves knockout controls or phosphatase treatment to confirm phosphorylation-dependent signals. By detecting Thr558 phosphorylation, this antibody provides insights into Rho/ROCK signaling pathways and their roles in cytoskeletal remodeling under physiological or disease contexts.
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