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产品描述：人可溶性血管内皮细胞蛋白C受体(sEPCR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PROCR含量。PROCR即蛋白C受体，在血管内皮细胞等表达，参与调节凝血与炎症反应。其研究机制主要围绕它与蛋白C结合，激活下游信号通路，发挥抗凝、抗炎及细胞保护作用，对维持血管稳态很关键，在心血管疾病等领域是潜在治疗靶点。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于心血管疾病机制研究、炎症反应模型构建、抗凝药物开发等科研领域。通过标准曲线定量分析，可精准测定不同处理条件下生物样本的sEPCR表达动态，为探索血栓性疾病病理过程及药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Activated protein C receptor ELISA Kit; APC receptor ELISA Kit; APCR ELISA Kit; bA42O4.2 ELISA Kit; CCCA ELISA Kit; CCD41 ELISA Kit; CD201 ELISA Kit; CD201 antigen ELISA Kit; cell cycle; centrosome-associated protein ELISA Kit; Cell cycle; centrosome-associated protein ELISA Kit; centrocyclin ELISA Kit; Endothelial cell protein C receptor ELISA Kit; Endothelial protein C receptor ELISA Kit; EPCR ELISA Kit; EPCR_HUMAN ELISA Kit; MGC23024 ELISA Kit; PROCR ELISA Kit; Protein C receptor; endothelial ELISA Kit
缩写：PROCR
Uniprot No.：Q9UNN8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sEPCR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human sEPCR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.8302.8122.8212.6822502.2122.2002.2062.0671251.4121.4361.4241.28562.50.7520.7120.7320.59331.20.3660.3840.3750.23615.60.2790.2610.2700.1317.80.2150.2000.2080.06900.1400.1380.139 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human EPCR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled EPCR antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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