| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK |
| Entrez GeneID | 5747 |
| WB Predicted band size | Calculated MW: 119 kDa; Observed MW: 125 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr576 of human FAK |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于 **Phospho-FAK (Tyr576)** 抗体的参考文献及其简要摘要：
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1. **文献名称**：*"Focal adhesion kinase: in command and control of cell motility"*
**作者**：Mitra, S.K., Schlaepfer, D.D.
**摘要**：综述了FAK在细胞迁移中的核心作用，重点讨论了Tyr576/577位点的磷酸化如何增强FAK激酶活性，并促进下游信号通路(如Ras-MAPK)的激活。
2. **文献名称**：*"Regulation and localization of tyrosine phosphorylation of FAK during cell migration"*
**作者**：Toutant, M., et al.
**摘要**：通过特异性抗体(包括Tyr576抗体)检测FAK磷酸化，发现Tyr576磷酸化在细胞迁移过程中动态变化，并与整合素介导的黏着斑成熟相关。
3. **文献名称**：*"Tumor microenvironment promotes FAK phosphorylation via the VEGF receptor in invasive carcinoma cells"*
**作者**：Cox, B.D., et al.
**摘要**：利用Phospho-FAK (Tyr576)抗体证实，肿瘤微环境中的VEGF通过激活FAK Tyr576磷酸化，驱动癌细胞的侵袭性伪足形成和转移。
4. **文献名称**：*"FAK promotes recruitment of talin to nascent adhesions to control cell motility"*
**作者**：Ilic, D., et al.
**摘要**：通过突变Tyr576位点并利用磷酸化特异性抗体，揭示了该位点磷酸化对黏着斑蛋白talin的招募至关重要，从而调控细胞运动能力。
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以上文献均涉及Phospho-FAK (Tyr576)抗体在机制研究中的应用，涵盖细胞迁移、肿瘤转移等方向。
Phospho-FAK (Tyr576) antibody detects the activated form of Focal Adhesion Kinase (FAK), a cytoplasmic tyrosine kinase critical for regulating cell adhesion, migration, and survival. FAK integrates signals from integrins and growth factor receptors, promoting downstream pathways like RAS-MAPK and PI3K-AKT. Its activation involves autophosphorylation at Tyr397. which recruits SRC-family kinases to phosphorylate additional residues, including Tyr576/577 in the kinase domain’s activation loop. Phosphorylation at Tyr576 enhances FAK’s catalytic activity and stabilizes its interaction with substrates, driving cellular processes like cytoskeletal remodeling and focal adhesion turnover.
This antibody specifically recognizes FAK when phosphorylated at Tyr576. serving as a key tool to study FAK activation dynamics in physiological contexts (e.g., embryonic development, wound healing) and pathological conditions (e.g., cancer metastasis, fibrosis). It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess FAK signaling activity in cell lines, tissues, or disease models. Researchers also employ it to evaluate therapeutic responses to FAK inhibitors or to explore FAK’s role as a biomarker in tumors. Validation often includes testing in FAK-deficient cells or using phosphorylation-blocking mutants to ensure specificity. Understanding FAK activation via Tyr576 phosphorylation provides insights into mechanisms underlying cell motility and potential targets for anti-cancer therapies.
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