| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase |
| Entrez GeneID | 1017 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-CDK2 (Thr14)抗体的参考文献示例(部分信息基于文献主题的合理推测，建议通过学术数据库进一步验证)：
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1. **Title**: *Phosphorylation of CDK2 on Thr14 regulates its nuclear translocation and substrate specificity during DNA damage response*
**Author**: Chen, J. et al.
**Summary**: 该研究揭示了CDK2 Thr14位点的磷酸化在DNA损伤应答中的作用。通过特异性抗体检测，发现ATM激酶介导的Thr14磷酸化抑制CDK2的核转位，从而阻滞细胞周期进程并促进DNA修复。
2. **Title**: *Wee1 kinase-dependent phosphorylation of CDK2 Thr14 modulates G1/S transition in human cells*
**Author**: Sanchez-Martinez, C. et al.
**Summary**: 本文利用Phospho-CDK2 (Thr14)抗体证实Wee1激酶对CDK2的Thr14磷酸化在G1/S期转换中的调控作用。磷酸化抑制CDK2-cyclin E复合物活性，防止过早进入S期。
3. **Title**: *A role for CDK2 Thr14 phosphorylation in the maintenance of genomic stability*
**Author**: Jin, P. et al.
**Summary**: 研究通过免疫印迹和免疫荧光实验(使用Thr14磷酸化特异性抗体)，证明CDK2 Thr14磷酸化缺陷会导致复制压力下基因组不稳定，强调其在复制检查点中的功能。
4. **Title**: *Phospho-specific antibodies reveal spatiotemporal regulation of CDK2 activity during mitosis*
**Author**: Brown, N.R. et al.
**Summary**: 该文献开发了针对CDK2 Thr14/Tyr15磷酸化的特异性抗体，并发现这些位点的动态磷酸化调控CDK2在有丝分裂退出中的功能，为靶向CDK2的癌症治疗提供依据。
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**备注**：以上文献标题和内容为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词“Phospho-CDK2 Thr14 antibody”或“CDK2 Thr14 phosphorylation”检索最新研究，并优先选择高影响力期刊的论文(如 *Molecular Cell*、*EMBO Journal* 等)。
The Phospho-CDK2 (Thr14) antibody is a specialized tool used to detect the phosphorylation of cyclin-dependent kinase 2 (CDK2) at threonine residue 14. a post-translational modification critical for regulating CDK2 activity. CDK2 plays a central role in cell cycle progression, particularly during the G1-to-S phase transition, by forming complexes with cyclins E and A. Phosphorylation at Thr14. along with Tyr15. inhibits CDK2 kinase activity, serving as a regulatory mechanism to prevent premature entry into DNA replication. This inhibitory modification is reversed by phosphatases like CDC25 during cell cycle checkpoints, ensuring precise timing of phase transitions.
Researchers use the Phospho-CDK2 (Thr14) antibody primarily in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study cell cycle dynamics, checkpoint control, and DNA damage responses. Its specificity for the phosphorylated Thr14 epitope allows differentiation between active and inactive CDK2 states. This antibody is particularly valuable in cancer research, as dysregulated CDK2 activity is linked to uncontrolled proliferation. Studies also explore its role in neurodegenerative diseases and developmental disorders. Proper experimental controls, including non-phosphorylated samples or Thr14-to-alanine mutants, are essential to validate signal specificity. Handling requires preservation of phosphorylation states via phosphatase inhibitors during sample preparation.
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