首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠催乳素(PRL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的PRL含量。PRL（催乳素）是一种由垂体前叶分泌的蛋白质激素，主要作用是促进乳腺发育和泌乳。研究发现，PRL及其受体PRLR在多种肿瘤中高表达，并促进肿瘤细胞增殖、血管生成和抵抗化疗。PRL-PRLR通路在乳腺癌、前列腺癌和胶质母细胞瘤等肿瘤中发挥作用，成为潜在的治疗靶点。试剂盒检测范围为0.125 ng/mL-50 ng/mL，适用于探究哺乳期动物模型、激素代谢异常机制、生殖生物学研究以及乳腺发育相关课题，特别在评估药物干预、环境因素或基因修饰对PRL分泌水平的影响实验中具有重要应用价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Prl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：PRL
Uniprot No.：P01237
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.125 ng/mL-50 ng/mL
灵敏度：0.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.1470.1460.147 9.370.3050.3140.310 1.560.5120.5230.518 0.440.8650.8870.876 0.1251.4111.4241.418 02.1182.1222.120 | ||||||
货期：3-5 working days
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