| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIF22; KID; KNSL4; Kinesin-like protein KIF22; Kinesin-like DNA-binding protein; Kinesin-like protein 4 |
| Entrez GeneID | 3835 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser427 of human KIF22 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-KIF22 (Ser427)抗体的3篇参考文献示例(注：部分内容为模拟生成，仅供参考)：
1. **文献名称**：*Phosphorylation of KIF22 at Ser427 regulates mitotic chromosome alignment and spindle dynamics*
**作者**：Li, X. et al.
**摘要**：本研究揭示了KIF22在Ser427位点的磷酸化对有丝分裂染色体排列和纺锤体稳定性的关键作用。通过Phospho-KIF22 (Ser427)抗体的免疫荧光分析，发现该磷酸化修饰通过调控KIF22与微管的结合能力，影响染色体运动与分裂进程。
2. **文献名称**：*KIF22 phosphorylation as a biomarker for hepatocellular carcinoma progression*
**作者**：Wang, Y. et al.
**摘要**：文章利用Phospho-KIF22 (Ser427)抗体检测肝癌组织中KIF22的磷酸化水平，发现其高表达与患者预后不良相关。功能实验表明，抑制Ser427磷酸化可阻断肿瘤细胞侵袭和转移。
3. **文献名称**：*CDK1-mediated phosphorylation of KIF22 at Ser427 drives glioma cell proliferation*
**作者**：Zhang, H. et al.
**摘要**：研究发现CDK1在胶质瘤细胞中磷酸化KIF22的Ser427位点，促进其与染色体结合。通过Phospho-KIF22 (Ser427)抗体的Western blot验证，该修饰与细胞周期异常和肿瘤生长密切相关。
4. **文献名称**：*Aurora kinase B-dependent phosphorylation of KIF22 modulates cytokinesis*
**作者**：Chen, J. et al.
**摘要**：本文证明Aurora kinase B通过磷酸化KIF22的Ser427位点调控胞质分裂。使用Phospho-KIF22 (Ser427)抗体进行活细胞成像，发现磷酸化缺失导致多核细胞形成，提示其在胞质分裂中的必要性。
(注：以上文献为示例性内容，实际文献需通过PubMed或SciHub等平台检索确认。)
Phospho-KIF22 (Ser427) antibody is a specialized tool used to detect the phosphorylated form of KIF22 at serine residue 427. KIF22. a member of the kinesin-like protein family, plays a critical role in mitotic processes, including chromosome alignment and spindle dynamics. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation. The phosphorylation of KIF22 at Ser427 is mediated by cyclin-dependent kinase 1 (CDK1) during mitosis, which is essential for its proper function in chromosome segregation. Dysregulation of KIF22 phosphorylation has been implicated in mitotic errors, genomic instability, and cancer progression.
This antibody is widely used in research to study cell cycle regulation, mitotic mechanisms, and cancer biology. It enables the detection of phosphorylated KIF22 in various applications, such as Western blotting, immunofluorescence, and immunohistochemistry. Researchers employ it to investigate how phosphorylation at Ser427 influences KIF22’s interaction with microtubules, its role in spindle assembly, and its potential as a biomarker in cancers where KIF22 is overexpressed or aberrantly activated. Specificity validation via knockout/knockdown controls or phosphatase treatment is recommended to confirm target recognition. Understanding KIF22 phosphorylation dynamics provides insights into mitotic regulation and therapeutic strategies targeting cell division abnormalities.
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