| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha |
| Entrez GeneID | 207 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr450 of human AKT1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-AKT1 (Thr450)抗体的参考文献示例(注：以下内容为虚构，供参考使用)：
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1. **文献名称**: *Phosphorylation of AKT1 at Thr450 Modulates Insulin Signaling in Hepatic Cells*
**作者**: Chen L, et al.
**摘要**: 本研究利用Phospho-AKT1 (Thr450)抗体揭示了该位点的磷酸化在胰岛素刺激下的动态变化，并证明其通过调控AKT1与PDK1的相互作用影响下游糖代谢通路。
2. **文献名称**: *Development and Validation of a Phospho-Specific Antibody Targeting AKT1 Thr450 for Cancer Research*
**作者**: Kumar S, et al.
**摘要**: 文章描述了一种新型Phospho-AKT1 (Thr450)抗体的开发流程，通过ELISA和免疫印迹验证其特异性，并应用于乳腺癌组织样本检测，发现与肿瘤侵袭性呈正相关。
3. **文献名称**: *Thr450 Phosphorylation of AKT1 Mediates Oxidative Stress Resistance in Neuronal Cells*
**作者**: Rodriguez E, et al.
**摘要**: 研究使用Phospho-AKT1 (Thr450)抗体证实，神经元中该位点的磷酸化通过激活Nrf2通路增强抗氧化能力，为神经退行性疾病提供了潜在治疗靶点。
4. **文献名称**: *AKT1 Thr450 Phosphorylation Dynamics During Cardiac Hypertrophy*
**作者**: Wang Y, et al.
**摘要**: 通过心肌细胞模型和Phospho-AKT1 (Thr450)抗体检测，发现压力超负荷诱导的心肌肥厚过程中，Thr450磷酸化水平显著上调，提示其参与病理性心脏重塑。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“Phospho-AKT1 Thr450 antibody”或结合具体研究领域进一步筛选。
**Background of Phospho-AKT1 (Thr450) Antibody**
The Phospho-AKT1 (Threonine 450) antibody is a specialized tool used to detect AKT1 protein phosphorylated at the Thr450 residue, a post-translational modification critical for regulating AKT1 activity. AKT1 (Protein Kinase B) is a serine/threonine kinase central to the PI3K/AKT/mTOR signaling pathway, which governs cellular processes such as survival, proliferation, metabolism, and apoptosis. Phosphorylation at Thr450 occurs in the catalytic domain of AKT1 and is associated with its maturation and stabilization. Unlike the canonical activation loop phosphorylation (Thr308/Ser473), Thr450 phosphorylation is not directly linked to immediate kinase activation but is thought to play a role in proper protein folding during AKT1 synthesis, ensuring its functional integrity.
This antibody is widely utilized in research to investigate AKT1 regulation, particularly in contexts of cancer, diabetes, and neurodegenerative diseases, where aberrant AKT signaling is often implicated. It enables the detection of phosphorylated AKT1 in various experimental setups, including Western blotting, immunofluorescence, and immunohistochemistry. Validation typically involves testing in cell lines with induced phosphorylation (e.g., growth factor stimulation) or genetic/pharmacologic inhibition. Researchers rely on this antibody to dissect AKT1 dynamics, assess pathway activation status, and evaluate therapeutic responses in preclinical models. Specificity for the Thr450 site ensures minimal cross-reactivity with other AKT isoforms (AKT2/AKT3) or unrelated phosphoproteins, making it a critical reagent for precision studies in signal transduction.
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