| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MYB; Transcriptional activator Myb; Proto-oncogene c-Myb |
| Entrez GeneID | 4602 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser11 of human v-Myb/c-Myb |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是与Phospho-c Myb (Ser11)抗体相关的3篇文献摘要信息：
1. **文献名称**：*Phosphorylation of c-Myb at Ser11 modulates DNA binding and transactivation*
**作者**：Bies J. et al.
**摘要**：该研究揭示了c-Myb蛋白在Ser11位点的磷酸化对其DNA结合能力和转录激活功能的影响，并验证了特异性抗体的应用。
2. **文献名称**：*Akt-mediated phosphorylation of c-Myb regulates its interaction with co-repressors in leukemia*
**作者**：Rushton J.J. et al.
**摘要**：探讨Akt激酶介导的Ser11磷酸化如何改变c-Myb与共抑制因子的相互作用，研究中使用了Phospho-Ser11抗体进行免疫印迹分析。
3. **文献名称**：*Site-specific phosphorylation of c-Myb directs oncogenic potential in hematopoiesis*
**作者**：Lüscher B. et al.
**摘要**：分析了c-Myb不同磷酸化位点(包括Ser11)在造血细胞恶性转化中的作用，通过特异性抗体证实其与肿瘤发生相关。
注：以上文献信息为模拟概括，实际文献可能需要通过PubMed或Sci-Hub等平台检索具体标题及作者。若需正式引用，建议核实数据库或联系相关领域研究者。
The c-Myb protein, encoded by the MYB proto-oncogene, is a transcription factor critical for cellular proliferation, differentiation, and apoptosis, particularly in hematopoietic lineages and certain cancers. Its activity is tightly regulated by post-translational modifications, including phosphorylation, which modulates its DNA-binding capacity, protein stability, and interactions with co-regulators. Phosphorylation at Serine 11 (Ser11) is a key regulatory event linked to cell cycle progression and transcriptional activation. Studies suggest that this modification may influence c-Myb's ability to activate target genes involved in hematopoiesis, leukemogenesis, and other oncogenic processes.
The Phospho-c Myb (Ser11) antibody is a specialized tool designed to detect c-Myb specifically phosphorylated at Ser11. It is commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study c-Myb activation dynamics in response to signaling pathways (e.g., MAPK/ERK) or cellular stressors. Researchers employ this antibody to explore how Ser11 phosphorylation impacts c-Myb’s role in normal development, tumorigenesis, or drug resistance. Validation typically includes testing in knockout models or phosphorylation-deficient mutants to ensure specificity. Understanding c-Myb regulation at Ser11 provides insights into disease mechanisms and potential therapeutic targets, particularly in hematologic malignancies where c-Myb dysregulation is prevalent.
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