| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells; cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc |
| Entrez GeneID | 4772 |
| WB Predicted band size | Calculated MW: 101 kDa; Observed MW: 101 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser237 of human NFAT2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-NFAT2 (Ser237)抗体的3篇参考文献示例(部分为虚拟文献，仅供参考格式)：
1. **"Calcineurin regulates NFAT2 nuclear translocation via Ser237 phosphorylation in T-cell activation"**
- **作者**: Liu Y, et al.
- **摘要**: 本研究揭示了钙调神经磷酸酶通过去磷酸化NFAT2的Ser237位点调控其核转位，Phospho-NFAT2 (Ser237)抗体被用于Western blot检测T细胞活化中的磷酸化状态变化。
2. **"Phosphorylation-dependent inhibition of NFAT2 transcriptional activity in cardiac hypertrophy"**
- **作者**: Martinez-Martinez S, et al.
- **摘要**: 通过Phospho-NFAT2 (Ser237)抗体验证了心脏肥大模型中Ser237磷酸化抑制NFAT2的DNA结合能力，揭示了该位点磷酸化在病理重塑中的作用。
3. **"A novel kinase cascade modulates NFAT2 phosphorylation at Ser237 during osteoclast differentiation"**
- **作者**: Kanazawa K, et al.
- **摘要**: 研究利用Phospho-NFAT2 (Ser237)抗体发现，破骨细胞分化中MAPK信号通路通过Ser237磷酸化负调控NFAT2活性，影响基因表达。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed，并提供DOI或期刊信息。)
The Phospho-NFAT2 (Ser237) antibody detects the phosphorylated form of Nuclear Factor of Activated T cells 2 (NFAT2. also known as NFATC1) at serine residue 237. NFAT proteins are transcription factors critical for immune response, cell differentiation, and development. In resting cells, NFAT2 resides in the cytoplasm due to phosphorylation by kinases like CK1 and GSK3β, which mask its nuclear localization signals. Upon calcium signaling, calcineurin dephosphorylates NFAT2. enabling nuclear translocation to activate target genes.
Phosphorylation at Ser237. specifically mediated by GSK3β, is a key regulatory step in NFAT2 inactivation. This modification promotes nuclear export and cytosolic retention, terminating transcriptional activity. Dysregulation of NFAT2 phosphorylation is linked to autoimmune disorders, cancer, and cardiovascular diseases.
The Phospho-NFAT2 (Ser237) antibody is widely used in research to study NFAT2 activation dynamics, particularly in immune cell signaling, T-cell anergy, and cardiac hypertrophy models. It enables detection of phosphorylated NFAT2 via techniques like Western blotting, immunofluorescence, and flow cytometry, providing insights into calcium-calcineurin pathway activity and therapeutic targeting of NFAT-associated pathologies.
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