| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 |
| Entrez GeneID | 3856 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser23 of human Cytokeratin 8 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-Cytokeratin 8 (Ser23)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Phosphorylation of Keratin 8 at Ser23 Modulates Hepatocyte Apoptosis*
**作者**：Oshima, R.G. et al.
**摘要**：研究揭示CK8在Ser23位点的磷酸化通过调节细胞骨架稳定性影响肝细胞凋亡，尤其在药物诱导的肝损伤中，磷酸化水平升高与caspase活化相关。
2. **文献名称**：*Phospho-Cytokeratin 8 (Ser23) as a Biomarker for Epithelial-Mesenchymal Transition in Colorectal Cancer*
**作者**：Feng, Y. et al.
**摘要**：该文献发现，结直肠癌中Ser23磷酸化的CK8表达上调与EMT进程相关，提示其可作为肿瘤侵袭性和转移潜能的标志物。
3. **文献名称**：*Stress-Induced Phosphorylation of Cytokeratin 8 at Ser23 Promotes Stress Granule Assembly*
**作者**：Liao, S.J. et al.
**摘要**：研究表明，氧化应激下CK8的Ser23磷酸化通过促进与RNA结合蛋白的相互作用，增强应激颗粒形成，从而调控细胞生存反应。
4. **文献名称**：*p38 MAPK-Mediated Phosphorylation of Cytokeratin 8 Regulates Cell Migration*
**作者**：Chen, X. et al.
**摘要**：该研究证实p38 MAPK通路介导的CK8 Ser23磷酸化通过改变细胞骨架动力学，增强上皮细胞的迁移能力，为癌症转移机制提供新见解。
(注：以上文献为示例，实际引用需查询具体数据库。)
Phospho-Cytokeratin 8 (Ser23) antibody detects the phosphorylated form of Cytokeratin 8 (CK8) at serine residue 23. a post-translational modification associated with cellular stress, apoptosis, and epithelial remodeling. Cytokeratin 8. encoded by the *KRT8* gene, is a type II intermediate filament protein predominantly expressed in simple and glandular epithelial cells. It pairs with type I keratins like CK18 to form a structural network that maintains cell integrity, mechanical stability, and organelle organization. Phosphorylation at Ser23 is linked to dynamic cytoskeletal reorganization during mitosis, apoptosis, or exposure to stressors like oxidative injury or chemotherapeutic agents. This modification is mediated by kinases such as p34cdc2 (CDK1), particularly during G2/M phase transitions, and may regulate filament disassembly or interactions with signaling proteins.
The Phospho-Cytokeratin 8 (Ser23) antibody is widely used in research to study epithelial cell responses to damage, tumor progression, and drug resistance. Elevated CK8 phosphorylation is observed in diseases like liver fibrosis, inflammatory bowel disease, and cancers (e.g., breast, colorectal), where it correlates with aggressive phenotypes. In diagnostic contexts, this antibody helps identify phosphorylated CK8 in tissue sections or cell lysates, aiding in the assessment of epithelial cell stress or proliferation states. Validation often includes Western blotting, immunofluorescence, or immunohistochemistry in models of apoptosis or epithelial injury. Its specificity for the phosphorylated epitope makes it a valuable tool for investigating intermediate filament dynamics and their role in disease mechanisms.
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