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Rabbit Monoclonal Phospho-AP2M1(Thr156) Antibody

  • 中文名： Phospho-AP2M1 (Thr156)抗体
  • 别    名： AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 subunit mu; Adaptin-mu2; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50
货号: IPDX20103
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 subunit mu; Adaptin-mu2; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50
Entrez GeneID1173
WB Predicted band sizeCalculated MW: 50 kDa; Observed MW: 50 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Thr156 of human AP2M1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-AP2M1 (Thr156)抗体的假设性参考文献示例(非真实文献，仅供格式参考)：

1. **文献名称**：*Phosphorylation of AP2M1 at Thr156 regulates clathrin-mediated endocytosis*

**作者**：Smith A, et al.

**摘要**：研究揭示了Thr156磷酸化通过抑制AP2复合体与货物蛋白的结合，调控网格蛋白介导的内吞过程，并开发了特异性抗体用于验证此修饰的功能影响。

2. **文献名称**：*Akt kinase phosphorylates AP2M1 to promote EGFR internalization in cancer cells*

**作者**：Chen L, et al.

**摘要**：报道Akt激酶介导的AP2M1 Thr156磷酸化增强了EGFR受体内吞，促进肿瘤细胞信号传导;研究使用Phospho-AP2M1 (Thr156)抗体进行免疫印迹和免疫荧光定位。

3. **文献名称**：*Dysregulated AP2M1 phosphorylation in neurodegenerative disease models*

**作者**：Wang Y, et al.

**摘要**：在阿尔茨海默病模型中，发现Thr156磷酸化水平异常升高，导致突触蛋白内吞障碍，该抗体被用于评估患者脑组织样本中的磷酸化状态。

4. **文献名称**：*Structural basis of AP2M1 phosphorylation-dependent cargo recognition*

**作者**：Johnson R, et al.

**摘要**：通过冷冻电镜解析AP2M1 Thr156磷酸化后的构象变化，阐明其如何阻碍衔接蛋白结合，研究依赖特异性抗体进行磷酸化位点验证。

(注：以上文献及内容为模拟生成，实际文献需通过PubMed/Google Scholar检索关键词如“AP2M1 phosphorylation Thr156”或“Phospho-AP2M1 antibody”获取。)

背景信息

The Phospho-AP2M1 (Thr156) antibody is a specialized tool used to detect the phosphorylation of the AP2M1 protein at threonine residue 156. AP2M1 (Adaptor-Related Protein Complex 2 Mu-1 subunit) is a critical component of the heterotetrameric AP-2 complex, which plays a central role in clathrin-mediated endocytosis. This process is essential for the internalization of transmembrane receptors, ion channels, and other cargo by facilitating vesicle formation at the plasma membrane. Phosphorylation of AP2M1 at Thr156 is a key post-translational modification that regulates AP-2’s ability to interact with cargo proteins and adaptor molecules, thereby modulating endocytic efficiency. Studies suggest that Thr156 phosphorylation may influence conformational changes in the AP-2 complex, impacting its recruitment to membranes or affinity for specific cargo motifs. This antibody is particularly useful in research exploring the molecular mechanisms of endocytosis, signal transduction, and membrane trafficking. It enables the identification of phosphorylation-dependent regulatory events in physiological processes such as synaptic vesicle recycling, receptor downregulation, and nutrient uptake. Dysregulation of AP2M1 phosphorylation has been implicated in diseases like cancer and neurological disorders, making this antibody a valuable tool for both basic and translational studies. Validation typically includes Western blotting and immunofluorescence to confirm specificity for the phosphorylated Thr156 epitope.

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