首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠嗜环蛋白/亲环素A(CyPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的PPIA含量。PPIA即亲环蛋白A，是一种广泛存在于各类细胞中的蛋白质。它参与众多细胞活动，如免疫调节、炎症反应等。研究机制方面，其可通过肽基脯氨酰顺反异构酶活性影响蛋白质折叠和功能，在疾病发生发展及药物研发中是重要靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于科研实验中探索CyPA在疾病模型中的表达调控机制、炎症相关信号通路研究以及药物干预效果评估等场景，为体外实验或动物模型中的生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ppia ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; PPIase A ELISA Kit; EC 5.2.1.8 ELISA Kit; Cyclophilin A ELISA Kit; Cyclosporin A-binding protein ELISA Kit; Rotamase A ELISA Kit; p1B15 ELISA Kit; p31) [Cleaved into: Peptidyl-prolyl cis-trans isomerase A ELISA Kit; N-terminally processed] ELISA Kit
缩写：PPIA
Uniprot No.：P10111
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CyPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 101-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 93-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 96-100 | |||||
回收率：
| The recovery of rat CyPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 106 | 103-109 | ||||
| EDTA plasma (n=4) | 92 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.7882.5952.6922.50940002.0121.9872.0001.81720001.3661.3231.3451.16210000.7890.7900.7900.6075000.5120.5260.5190.3362500.3890.3820.3860.2031250.2540.2640.2590.07600.1800.1860.183 | ||||||
货期：3-5 working days
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