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产品描述：人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的PPARGC1A含量。PPARGC1A是一种转录共激活子，调控能量代谢相关基因的表达。它与PPARgamma相互作用，参与调节线粒体生物发生、能量代谢和热生成等过程。研究显示，PPARGC1A在多种生理和病理过程中发挥重要作用，如缺血性中风和前列腺癌。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于探索能量代谢通路、疾病模型（如糖尿病、肥胖、阿尔茨海默病等）中PPARGC1α的表达动态，或评估药物干预对线粒体功能的影响；为分子机制研究、生物标志物筛选等科研领域提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：L PGC 1alpha ELISA Kit; LEM6 ELISA Kit; Ligand effect modulator 6 ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC 1 (alpha) ELISA Kit; PGC 1 alpha ELISA Kit; PGC 1v ELISA Kit; PGC-1-alpha ELISA Kit; PGC1 ELISA Kit; PGC1(alpha) ELISA Kit; PGC1A ELISA Kit; PGC1v ELISA Kit; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 ELISA Kit; PPAR gamma coactivator 1 alpha ELISA Kit; PPAR gamma coactivator 1 ELISA Kit; PPAR gamma coactivator variant form ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC 1 alpha ELISA Kit; PPARGC-1-alpha ELISA Kit; PPARGC1 ELISA Kit; PPARGC1A ELISA Kit; PRGC1_HUMAN ELISA Kit
缩写：PPARGC1A
Uniprot No.：Q9UBK2
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPARGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human PPARGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.0542.0522.0531.95440001.3151.3251.3201.22120000.7620.7770.7700.67110000.4700.4770.4740.3755000.2840.2950.2900.1912500.2160.2240.2200.1211250.1570.1650.1610.06200.0960.1020.099 | ||||||
货期：3-5 working days
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