首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠过氧化物酶体增殖因子活化受体γ( PPAR-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPARG含量。PPARG即过氧化物酶体增殖物激活受体γ，在脂质代谢、糖稳态维持及细胞分化等过程中发挥关键作用。研究发现其可与配体结合调节靶基因表达，在改善胰岛素敏感性等方面机制明确，是糖尿病等代谢疾病药物研发的热门靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研实验中探索PPAR - γ在肥胖、胰岛素抵抗等病理生理过程中的表达特征，或用于评估药物干预、基因编辑等处理对靶蛋白表达的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pparg ELISA Kit; Nr1c3 ELISA Kit; Peroxisome proliferator-activated receptor gamma ELISA Kit; PPAR-gamma ELISA Kit; Nuclear receptor subfamily 1 group C member 3 ELISA Kit
缩写：PPARG
Uniprot No.：O88275
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PPAR-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 100-109 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat PPAR-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-95 | ||||
| EDTA plasma (n=4) | 98 | 93-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3712.3032.3372.23951.7121.8261.7691.6712.51.1571.1141.1361.0381.250.6040.5760.5900.4920.6250.3120.3270.3200.2220.3120.1790.1690.1740.0760.1560.1150.1090.1120.01400.0940.1010.098 | ||||||
货期：3-5 working days
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