| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOMM34 |
| Uniprot No | Q15785 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAPKFPD SVEELRAAGN ESFRNGQYAE ASALYGRALR VLQAQGSSDP EEESVLYSNR AACHLKDGNC RDCIKDCTSA LALVPFSIKP LLRRASAYEA LEKYPMAYVD YKTVLQIDDN VTSAVEGINR MTRALMDSLG PEWRLKLPSI PLVPVSAQKR WNSLPSENHK EMAKSKSKET TATKNRVPSA GDVEKARVLK EEGNELVKKG NHKKAIEKYS ESLLCSNLES ATYSNRALCY LVLKQYTEAV KDCTEALKLD GKNVKAFYRR AQAHKALKDY KSSFADISNL LQIEPRNGPA QKLRQEVKQN LHKDCTEALKLD GKNVKAFYRR AQAHKALKDY KSSFADISNL LQIEPRNGPA QKLRQEVKQN LH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOMM34重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Structural and Functional Analysis of TOMM34 Reveals a Novel Mitochondrial Protein Import Regulatory Mechanism*
**作者**：Yamano, K. 等 (2018)
**摘要**：通过重组TOMM34蛋白的晶体结构解析，研究揭示了其作为分子伴侣在线粒体蛋白输入中的作用，发现其与Hsp70协同调控底物蛋白的识别与转运。
2. **文献名称**：*TOMM34 Promotes Hepatocellular Carcinoma Metastasis via Stabilizing EGFR and Activating PI3K/AKT Pathway*
**作者**：Li, X. 等 (2020)
**摘要**：利用重组TOMM34蛋白进行功能实验，发现其通过结合并稳定EGFR蛋白，激活PI3K/AKT信号通路，从而促进肝癌细胞侵袭转移。
3. **文献名称**：*Recombinant TOMM34 Modulates Mitochondrial Dynamics and Enhances Neuronal Survival in Parkinson’s Disease Models*
**作者**：Suzuki, T. 等 (2019)
**摘要**：研究显示，重组TOMM34蛋白通过调控线粒体分裂-融合平衡，减少多巴胺能神经元损伤，为帕金森病的治疗提供潜在靶点。
注：以上文献信息为示例性质，具体内容需根据实际发表论文调整。建议通过PubMed或Web of Science以“TOMM34 recombinant”为关键词检索最新研究。
TOMM34 (Translocase of Outer Mitochondrial Membrane 34) is a protein encoded by the TOMM34 gene, primarily localized to the outer mitochondrial membrane. It plays a regulatory role in mitochondrial protein import, acting as a co-chaperone that assists in the assembly and stability of the TOM (Translocase of the Outer Mitochondrial Membrane) complex. Unlike core TOM components (e.g., TOMM20 or TOMM40), TOMM34 does not directly form the protein-conducting channel but interacts with cytosolic chaperones like Hsp70/Hsp90 to facilitate the delivery of precursor proteins to mitochondria. This process is critical for maintaining mitochondrial function, particularly in energy production, apoptosis, and cellular stress responses.
TOMM34 is overexpressed in various cancers, including colorectal, pancreatic, and hepatocellular carcinomas, where it correlates with poor prognosis. Its role in cancer progression is linked to enhanced mitochondrial biogenesis, evasion of apoptosis, and metabolic reprogramming. Additionally, TOMM34 has been implicated in neurodegenerative diseases, such as Alzheimer’s, due to its involvement in mitochondrial dysfunction.
Recombinant TOMM34 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, interactions, and mechanisms. Researchers use it to investigate mitochondrial import pathways, screen for inhibitors in cancer therapy, or develop diagnostic biomarkers. Its recombinant form retains binding affinity for chaperones and precursor proteins, making it a valuable tool for *in vitro* assays and structural studies. Understanding TOMM34’s molecular dynamics could unveil therapeutic strategies targeting mitochondrial dysfunction in disease contexts.
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