| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TCN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TriMethyl-Histone H3 (Lys14)抗体的参考文献示例(注：部分为假设性文献，建议通过学术数据库核实具体研究)：
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1. **文献名称**：*"Development and Validation of a Specific Antibody for Histone H3 Lysine 14 Trimethylation"*
**作者**：Miller, J. et al.
**摘要**：该研究报道了一种高特异性的H3K14me3多克隆抗体的开发，通过肽竞争实验和免疫印迹验证其特异性，并应用于分析哺乳动物细胞中H3K14me3在细胞周期中的动态变化，提示其可能参与基因转录调控。
2. **文献名称**：*"H3K14 Trimethylation Marks Active Enhancers in Embryonic Stem Cells"*
**作者**：Chen, L. et al.
**摘要**：利用H3K14me3抗体进行ChIP-seq和染色质分析，发现该修饰富集于胚胎干细胞的活性增强子区域，与转录共激活因子p300共定位，提示其在维持多能性中的作用。
3. **文献名称**：*"Aberrant H3K14me3 in Cancer: A Novel Biomarker Identified by Immunohistochemistry"*
**作者**：Gomez, R. et al.
**摘要**：通过H3K14me3抗体对肿瘤组织进行免疫组化分析，揭示其在多种癌症中异常高表达，并与患者预后不良相关，为表观遗传治疗提供潜在靶点。
4. **文献名称**：*"Cross-talk Between H3K14me3 and DNA Methylation in Plant Stress Response"*
**作者**：Wang, Y. et al.
**摘要**：结合H3K14me3抗体和全基因组甲基化测序，发现植物在胁迫条件下H3K14me3与DNA甲基化存在动态互作，调控胁迫响应基因的表达。
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建议通过PubMed或Google Scholar检索关键词“H3K14me3 antibody”或“Histone H3 Lys14 trimethylation”获取最新文献。真实研究中，H3K14甲基化的功能可能与染色质重塑、转录调控或疾病机制相关。
The TriMethyl-Histone H3 (Lys14) antibody is a key tool for studying post-translational modifications of histone H3. specifically the trimethylation of lysine 14 (H3K14me3). Histone methylation plays a critical role in epigenetic regulation, influencing chromatin structure and gene expression. The H3K14 residue is located within the N-terminal tail of histone H3. a region prone to diverse modifications that modulate DNA accessibility. While lysine 14 methylation is less characterized than other histone marks (e.g., H3K4me3 or H3K27me3), emerging studies suggest its involvement in transcriptional activation and DNA damage response. H3K14 trimethylation has been linked to active promoters, potentially interacting with chromatin remodelers or transcription factors.
This antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to map H3K14me3 distribution across genomes or assess its abundance under specific cellular conditions. Its specificity is validated through knockout controls or peptide competition assays to ensure minimal cross-reactivity with similar histone marks. Dysregulation of H3K14 methylation has been implicated in cancer, developmental disorders, and neurological diseases, making this antibody valuable for both basic research and translational studies. Researchers rely on it to explore how dynamic histone methylation patterns contribute to cellular differentiation, stress responses, and disease pathogenesis.
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