| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOM1L2 |
| Uniprot No | Q6ZVM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFMEFLLGNPFSTPVGQCLEKATDGSL QSEDWTLNMEICDIINETEEGPKDAIRALKKRLNGNRNYREVMLALTVLE TCVKNCGHRFHILVANRDFIDSVLVKIISPKNNPPTIVQDKVLALIQAWA DAFRSSPDLTGVVHIYEELKRKGVEFPMADLDALSPIHTPQRSVPEVDPA ATMPRSQSQQRTSAGSYSSPPPAPYSAPQAPALSVTGPITANSEQIARLR SELDVVRGNTKVMSEMLTEMVPGQEDSSDLELLQELNRTCRAMQQRIVEL ISRVSNEEVTEELLHVNDDLNNVFLRYERFERYRSGRSVQNASNGVLNEV TEDNLIDLGPGSPAVVSPMVGNTAPPSSLSSQLAGLDLGTESVSGTLSSL QQCNPRDGFDMFAQTRGNSLAEQRKTVTYEDPQAVGGLASALDNRKQSSE GIPVAQPSVMDDIEVWLRTDLKGDDLEEGVTSEEFDKFLEERAKAAEMVP DLPSPPMEAPAPASNPSGRKKPERSEDALFAL |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOM1L2重组蛋白的3篇参考文献示例(注：内容为假设性概括，实际文献需通过学术数据库检索获取)：
1. **文献名称**：*"Cloning and Characterization of Recombinant Human TOM1L2 Protein"*
**作者**：Smith A, et al.
**摘要**：本研究成功克隆了人源TOM1L2基因，并在大肠杆菌系统中实现重组表达。通过亲和层析纯化获得高纯度蛋白，并验证其与细胞内吞作用相关蛋白的相互作用，为后续功能研究奠定基础。
2. **文献名称**：*"Structural Insights into TOM1L2 in Ubiquitin-Dependent Trafficking Pathways"*
**作者**：Lee J, Brown K.
**摘要**：利用重组TOM1L2蛋白进行X射线晶体学分析，揭示了其VHS结构域与泛素化信号的特异性结合模式，阐明其在溶酶体运输通路中的分子机制。
3. **文献名称**：*"TOM1L2 Modulates EGFR Signaling through Recombinant Protein Interaction Studies"*
**作者**：Garcia R, et al.
**摘要**：通过体外重组TOM1L2蛋白与EGFR的共沉淀实验，发现其通过调控受体酪氨酸激酶的内吞降解影响癌细胞信号转导，提示其作为潜在治疗靶点。
如需真实文献，建议在PubMed或Web of Science中搜索关键词“TOM1L2 recombinant protein”获取最新研究。
TOM1L2 (Target Of Myb1-like protein 2) is a member of the TOM1 family of adaptor proteins involved in intracellular trafficking and signal transduction. It shares structural homology with other TOM1 family members, including a VHS domain (Vps27. Hrs, STAM), a GAT domain (GGA and TOM1), and a C-terminal clathrin-binding motif. These domains enable interactions with ubiquitinated proteins, clathrin, and other trafficking machinery components. TOM1L2 is implicated in endosomal sorting, particularly in regulating the lysosomal degradation pathway by linking ubiquitinated cargo to the ESCRT (Endosomal Sorting Complex Required for Transport) system.
Functionally, TOM1L2 interacts with ubiquitinated receptors and signaling molecules, influencing processes like receptor turnover, immune response modulation, and growth factor signaling. Studies suggest its role in cancer progression, as overexpression correlates with enhanced cell migration and metastasis in certain malignancies. It also associates with Nedd4 family E3 ubiquitin ligases, which are critical in protein quality control and degradation.
Recombinant TOM1L2 proteins are engineered to study these mechanisms *in vitro* or *in vivo*. They are typically expressed in bacterial (e.g., *E. coli*) or mammalian systems, often tagged with fluorescent markers (e.g., GFP) or affinity tags (e.g., His-tag) for purification and tracking. Research using recombinant TOM1L2 has clarified its PTAP motif-mediated binding to ubiquitin and its role in viral pathogenesis, as some pathogens exploit endocytic pathways involving TOM1L2. Ongoing studies explore its potential as a therapeutic target, particularly in diseases with dysregulated protein trafficking, such as neurodegenerative disorders and cancers.
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