| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H4K20me3; H4F2; HIST; HIST1H4A; Histone H4 |
| Entrez GeneID | 121504 |
| WB Predicted band size | Calculated MW: 11 kDa; Observed MW: 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H4 (tri methyl K20) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与H4K20me3(TriMethyl-Histone H4 Lys20)抗体相关的经典文献，涵盖功能研究和应用方向：
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1. **文献名称**: *The role of H4K20 methylation in DNA repair and heterochromatin formation*
**作者**: Beck DB, et al.
**摘要**: 该研究通过H4K20me3特异性抗体的ChIP-seq分析，揭示了该修饰在DNA损伤修复中的关键作用，并证明其与53BP1蛋白结合，参与维持基因组稳定性(Nature, 2012)。
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2. **文献名称**: *Drosophila Tridax regulates H4K20 trimethylation-mediated heterochromatin formation*
**作者**: Sakaguchi A, Steward R.
**摘要**: 作者利用H4K20me3抗体进行免疫荧光和Western blot，发现果蝇中H4K20三甲基化对异染色质组装至关重要，并影响基因沉默(Genes & Development, 2007)。
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3. **文献名称**: *A chromatin-wide transition to H4K20 trimethylation during differentiation*
**作者**: Schotta G, et al.
**摘要**: 通过H4K20me3抗体的全基因组分析，揭示了哺乳动物细胞分化过程中该修饰的动态变化及其在染色质压缩和转录抑制中的调控机制(Nature Cell Biology, 2004)。
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4. **文献名称**: *H4K20me3 regulates mitotic condensation and mitotic exit*
**作者**: Jørgensen S, et al.
**摘要**: 研究使用H4K20me3抗体结合活细胞成像，证明该修饰通过招募CDK1调控有丝分裂染色质凝聚及细胞周期进程(Science, 2013)。
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这些文献覆盖了H4K20me3在表观遗传调控、DNA修复和细胞周期中的关键功能，并展示了相关抗体的实验应用场景(如ChIP-seq、免疫荧光等)。如需具体DOI或补充近5年文献，可进一步说明。
TriMethyl-Histone H4 (Lys20) [H4K20me3] antibodies are essential tools for studying post-translational modifications of histone H4. specifically trimethylation at lysine 20. This epigenetic mark plays a critical role in chromatin organization and genome regulation. H4K20me3 is predominantly associated with constitutive heterochromatin, contributing to chromosomal stability, DNA repair, and transcriptional silencing. It is enriched in repetitive regions, centromeres, and telomeres, where it facilitates the recruitment of proteins like HP1 (heterochromatin protein 1) and Crb2. which mediate chromatin compaction and DNA damage response.
The antibody detects the trimethylated form of lysine 20. distinguishing it from mono- or dimethylated states (H4K20me1/me2), which have distinct biological roles. H4K20me1 is linked to transcriptional activation, while H4K20me2 is broadly distributed across euchromatin. Dysregulation of H4K20me3 has been implicated in cancer, developmental disorders, and aging. For example, reduced H4K20me3 levels are observed in various malignancies, correlating with genomic instability and oncogenesis.
Researchers use H4K20me3-specific antibodies in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to map heterochromatin domains, study cell cycle-dependent chromatin changes, or investigate epigenetic alterations in disease models. Commercial antibodies are typically validated for specificity using knockout cell lines or peptide competition assays. Understanding H4K20me3 dynamics provides insights into epigenetic mechanisms governing genome integrity and cellular identity.
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