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产品描述：人胎盘特异性蛋白1(PLAC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAC1含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，本试剂盒基于高特异性配对抗体建立，适用于体外定量检测多种生物样本中PLAC1的浓度变化，为研究其在生殖生物学、肿瘤发生机制中的功能提供可靠工具，亦可用于探索PLAC1作为特定疾病模型的潜在生物标志物。实验操作兼容常规酶标仪检测，支持科研人员开展细胞实验、动物模型及离体组织样本的相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cancer/testis antigen 92 ELISA Kit; CT92 ELISA Kit; Plac1 ELISA Kit; PLAC1_HUMAN ELISA Kit; Placenta specific 1 ELISA Kit; Placenta-specific protein 1 ELISA Kit
缩写：PLAC1
Uniprot No.：Q9HBJ0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-89 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human PLAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6122.6512.6322.420501.9072.0131.9601.748251.1951.2361.2161.00412.50.7660.7820.7740.5626.250.5050.5160.5110.2993.130.3730.3840.3790.1671.560.3100.3160.3130.10100.2090.2140.212 | ||||||
货期：3-5 working days
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