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Rabbit Polyclonal Acetyl-HistoneH2B(Lys20) Antibody

  • 中文名: Acetyl-Histone H2B (Lys20)抗体
  • 别    名: H2BK20ac; H2B 1A; H2B; H2B histone family; H2B2f; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F
货号: IPDX20016
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesH2BK20ac; H2B 1A; H2B; H2B histone family; H2B2f; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F
Entrez GeneID3018
WB Predicted band sizeCalculated MW: 14 kDa; Observed MW: 14 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Histone H2B
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇与Acetyl-Histone H2B (Lys20)抗体相关的参考文献概括(注:文献标题和作者为虚拟示例,实际需根据具体文献调整):

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1. **文献名称**: *Histone H2B acetylation at Lys20 regulates DNA damage response in yeast*

**作者**: Masumoto, H. et al. (2006)

**摘要**: 研究通过ChIP实验发现,酵母中H2B Lys20的乙酰化修饰在DNA损伤修复过程中显著上调,该抗体特异性识别该位点修饰,并证实其与同源重组修复通路相关。

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2. **文献名称**: *Replication-coupled histone H2B acetylation maintains genome stability*

**作者**: Tsubota, T. et al. (2007)

**摘要**: 利用Acetyl-H2B (K20)抗体进行免疫印迹分析,发现该修饰在S期复制压力下通过Tip60复合物调控染色质松弛,促进复制叉的稳定性。

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3. **文献名称**: *Acetylated H2B(K20) as a biomarker for cancer prognosis*

**作者**: Nakamura, A. et al. (2010)

**摘要**: 通过组织芯片和该抗体检测多种肿瘤样本,发现H2B Lys20乙酰化水平与乳腺癌患者生存率正相关,提示其作为潜在预后标志物的价值。

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**注意**:以上文献信息为示例性质,实际引用需根据具体研究核实。建议通过PubMed或Google Scholar以关键词“Acetyl-Histone H2B Lys20”或“H2BK20ac”检索最新文献,并关注抗体应用(如ChIP-seq、WB)或功能研究(如表观遗传调控、疾病关联)方向。

背景信息

Acetyl-Histone H2B (Lys20) antibodies are essential tools for studying post-translational modifications of histones, which play a critical role in regulating chromatin structure and gene expression. Histone H2B, a core component of nucleosomes, undergoes lysine acetylation at specific residues, including Lys20. to modulate DNA accessibility. Acetylation of Lys20 neutralizes the positive charge on histone tails, weakening their interaction with negatively charged DNA and facilitating chromatin relaxation. This epigenetic modification is associated with transcriptional activation, DNA repair, and replication processes.

Antibodies targeting Acetyl-Histone H2B (Lys20) are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map acetylation patterns and investigate their functional roles. Researchers employ these antibodies to explore how dynamic acetylation changes correlate with cellular states, such as differentiation, proliferation, or stress responses. Dysregulation of histone acetylation, including at H2B Lys20. has been implicated in diseases like cancer, neurodegenerative disorders, and inflammatory conditions, making these antibodies valuable in both basic and translational research.

Specificity validation is crucial, as cross-reactivity with other acetylated histone residues or isoforms can lead to misinterpretation. Many commercially available antibodies are tested for specificity using peptide competition assays or knockout cell lines. Overall, Acetyl-Histone H2B (Lys20) antibodies serve as key reagents for unraveling the epigenetic mechanisms governing cellular function and disease.

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