| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H4K5ac; H4F2; HIST; HIST1H4A; Histone H4.AC-H4K5; H4K5 |
| Entrez GeneID | 121504 |
| WB Predicted band size | Calculated MW: 11 kDa; Observed MW: 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Histone H4 (acetyl K5) |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及Acetyl-Histone H4 (Lys5)抗体的研究文献示例(文献信息为虚拟概括，仅供格式参考)：
1. **"Histone H4 lysine 5 acetylation regulates transcriptional initiation in mammalian cells"**
*作者：Smith et al.*
摘要：该研究利用H4K5ac特异性抗体进行ChIP-seq分析，揭示了H4K5乙酰化在启动子区域的富集与RNA聚合酶II招募的关联性，表明其在转录起始中的调控作用。
2. **"Dynamic acetylation of H4K5 during DNA damage response"**
*作者：Lee & Thompson*
摘要：通过免疫荧光和Western blotting，研究者发现H4K5乙酰化水平在DNA双链断裂后显著升高，提示其参与DNA损伤修复的信号通路激活。
3. **"Epigenetic regulation of stem cell differentiation by H4K5 acetylation"**
*作者：Zhang et al.*
摘要：使用H4K5ac抗体分析胚胎干细胞分化过程，发现该修饰通过调控多能性基因(如Oct4)的染色质开放性影响细胞命运决定。
4. **"Aberrant H4K5 acetylation as a biomarker in breast cancer progression"**
*作者：Chen et al.*
摘要：研究通过组织芯片免疫组化检测，证明H4K5ac在乳腺癌组织中异常高表达，并与患者预后不良相关，可能成为潜在治疗靶点。
(注：以上文献为示例性内容，实际引用需查询真实数据库如PubMed。)
Acetyl-Histone H4 (Lys5) antibodies are essential tools for studying epigenetic regulation linked to histone acetylation, a post-translational modification critical for chromatin structure and gene expression. Histone H4 acetylation at lysine 5 (H4K5ac) is associated with relaxed chromatin states, facilitating transcriptional activation by promoting accessibility of DNA to transcription factors and RNA polymerase. This modification is dynamically regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs), which balance acetylation levels to control cellular processes like DNA replication, repair, and differentiation.
H4K5ac often cooperates with other acetylated lysine residues (e.g., H4K8. H4K12. H4K16) to establish specific acetylation patterns that influence nucleosome stability and transcriptional activity. Studies suggest H4K5ac is enriched at active gene promoters and enhancers, serving as a marker for regulatory regions. Dysregulation of H4K5ac has been implicated in diseases such as cancer, neurodegeneration, and metabolic disorders, making its detection crucial for understanding disease mechanisms.
These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map acetylation sites, assess epigenetic states, and evaluate drug effects targeting HDACs or HATs. Validated for specificity, they help researchers explore how H4K5ac contributes to cellular identity, stress responses, and therapeutic interventions in epigenetic diseases.
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