| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NUDT19; RP2;;NUDT19 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NUDT19 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NUDT19抗体的模拟参考文献示例(注：部分内容基于文献推测，建议通过学术数据库核实真实文献)：
1. **文献名称**：*"Characterization of a Novel NUDT19 Antibody for Detecting Tissue-Specific Expression Patterns"*
**作者**：Li, X.; Zhang, Y.; Wang, J.
**摘要**：该研究开发了一种高特异性兔源多克隆抗体，用于检测NUDT19蛋白在小鼠肝脏和肾脏中的表达，验证了其在Western blot和免疫组化中的应用，揭示了NUDT19在脂质代谢相关组织中的分布特征。
2. **文献名称**：*"NUDT19's Role in Coenzyme A Metabolism Revealed by CRISPR/Cas9 and Antibody-Based Knockdown Studies"*
**作者**：Tanaka, K.; Ito, S.
**摘要**：通过结合CRISPR/Cas9基因编辑和特异性抗体介导的蛋白敲低技术，研究发现NUDT19通过水解CoA衍生物调控细胞能量代谢，抗体被用于验证基因敲除后蛋白表达水平的变化。
3. **文献名称**：*"Structural Insights into NUDT19 Substrate Specificity Using Epitope-Specific Monoclonal Antibodies"*
**作者**：Guo, R.; Chen, L.
**摘要**：研究团队利用单克隆抗体进行免疫共沉淀实验，解析了NUDT19与底物结合的构象变化，证实其偏好水解长链酰基-CoA底物，抗体在结构生物学分析中发挥了关键作用。
4. **文献名称**：*"Development of a NUDT19 Knockout Mouse Model Validated by Immunohistochemistry and Phenotypic Analysis"*
**作者**：Miller, A.; Johnson, R.
**摘要**：通过构建NUDT19基因敲除小鼠模型，并利用定制抗体进行组织染色，发现NUDT19缺失导致肝脏脂质异常累积，提示其在脂类稳态中的潜在功能。
**注意**：以上文献为模拟内容，实际研究请查询PubMed、Google Scholar等平台获取最新数据。
The NUDT19 antibody is a research tool designed to detect and study the NUDT19 protein, a member of the Nudix hydrolase family. NUDT19. also known as nudix hydrolase 19. is an enzyme encoded by the *NUDT19* gene in humans. It is primarily localized to peroxisomes and mitochondria, where it catalyzes the hydrolysis of nucleotide diphosphate derivatives, including Coenzyme A (CoA) esters, NADH, and NADPH. This enzymatic activity suggests its role in regulating cellular redox balance, lipid metabolism, and detoxification processes. Dysregulation of NUDT19 has been implicated in metabolic disorders and certain cancers, though its precise physiological and pathological mechanisms remain under investigation.
The NUDT19 antibody is typically generated using immunogenic peptides or recombinant protein fragments, enabling specificity in detecting endogenous NUDT19 across applications like Western blotting, immunohistochemistry, and immunofluorescence. Its development supports efforts to map NUDT19 expression patterns, subcellular localization, and interactions in various tissues and disease models. Recent studies highlight its potential relevance in peroxisomal CoA degradation pathways and lipid homeostasis, making it a valuable reagent for exploring metabolic diseases, cancer biology, and drug discovery. However, validation of antibody specificity using knockout controls is critical due to structural similarities within the Nudix family.
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