| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NPAP60; NPAP60L; Nucleoporin 50kDa; Nup50;;NUP50 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NUP50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于NUP50抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*NUP50 is essential for cell cycle progression and spatial organization of the nuclear pore complex*
**作者**：Bastos, R. et al.
**摘要**：该研究通过使用特异性NUP50抗体，揭示了NUP50在维持核孔复合体(NPC)结构和调控细胞周期中的关键作用。实验表明，NUP50缺失导致有丝分裂异常和核膜重组缺陷，抗体用于免疫荧光定位其在细胞分裂中的动态变化。
2. **文献名称**：*A role for NUP50 in modulating nuclear transport and genome integrity*
**作者**：Griffis, E.R. et al.
**摘要**：作者利用NUP50抗体进行免疫共沉淀和蛋白质组学分析，发现NUP50通过调控mRNA出核运输参与基因组稳定性维持。抗体验证了其在多种细胞系中的表达及与其他核转运因子的相互作用。
3. **文献名称**：*Characterization of a monoclonal antibody against NUP50 for studying nuclear pore dynamics in cancer cells*
**作者**：Chen, L. et al.
**摘要**：本文报道了一种高特异性抗NUP50单克隆抗体的开发，并通过Western blot和免疫电镜验证其可靠性。研究发现NUP50在多种癌细胞中表达上调，可能成为肿瘤诊断的潜在标志物。
4. **文献名称**：*NUP50 regulates selective chromatin interactions and transcriptional activation*
**作者**：Kalverda, B. et al.
**摘要**：研究使用NUP50抗体进行ChIP-seq分析，揭示其通过介导染色质与核孔的结合影响基因转录活性，尤其在胚胎干细胞分化中调控多能性相关基因的表达。
(注：上述文献信息为示例性整合，实际引用需根据具体论文调整。)
The NUP50 antibody is a crucial tool for studying the nuclear pore complex (NPC) and nucleocytoplasmic transport mechanisms. NUP50 (Nucleoporin 50), also known as NPAP60. is a dynamic component of the NPC, a large protein assembly embedded in the nuclear envelope. Unlike many structural nucleoporins, NUP50 localizes primarily to the nuclear basket and exhibits mobile behavior, facilitating selective transport of macromolecules between the nucleus and cytoplasm. It plays roles in mRNA export, mitotic progression, and chromatin organization by interacting with transport receptors (e.g., importin-β) and other nucleoporins like NUP153 and RAE1.
NUP50 antibodies are widely used in immunofluorescence, Western blotting, and immunoprecipitation to investigate its spatial distribution, protein interactions, and expression changes during cellular processes. These antibodies often target conserved regions, such as the C-terminal domain containing FG (phenylalanine-glycine) repeats critical for transport functions. Researchers validate specificity using knockout cell lines or siRNA-mediated knockdown. Commercial NUP50 antibodies are typically raised in rabbits or mice, with clones validated for cross-reactivity in human, mouse, and rat models.
Dysregulation of NUP50 is linked to diseases including cancers and infertility, making its antibody a key reagent for studying NPC-related pathologies. Recent studies also explore its non-canonical roles in DNA repair and viral infection, expanding its research relevance.
×
