首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前列腺素E2(PGE2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PG-E2含量。PG-E2（前列腺素 E2）是一种生物活性脂质介质。它在炎症反应、免疫调节、组织修复等多种生理和病理过程中发挥重要作用。其研究机制主要聚焦于与特定受体结合，激活细胞内信号通路，从而影响细胞的增殖、分化和功能，对相关疾病治疗研究有重要意义。试剂盒检测范围为1.5 pg/mL-300 pg/mL，本试剂盒可为体外研究炎症介导机制、免疫调节通路、药物干预效果评估等提供可靠数据支持，特别适用于实验室条件下探索生物样本中PGE2的动态变化规律，满足细胞实验、动物模型或离体组织分析等基础科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PG-E2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.5 pg/mL-300 pg/mL
灵敏度：0.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 90 | 83-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 3000.1930.2010.197 750.3490.3270.338 240.5230.5310.527 60.9030.9620.933 1.51.3161.3931.355 02.2712.2122.242 | ||||||
货期：3-5 working days
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