首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人平足蛋白(PDPN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDPN含量。PDPN 即血小板反应蛋白 4 结合蛋白，是一种跨膜黏蛋白。它在肿瘤、淋巴管生成等方面扮演重要角色。其研究机制主要围绕它与配体相互作用，参与肿瘤细胞的增殖、迁移和转移，也会影响免疫细胞功能，是肿瘤治疗等领域的潜在靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，科研人员可将其用于探索PDPN在肿瘤微环境调控、免疫细胞功能研究或心血管疾病模型中的作用机制，亦可通过检测不同处理条件下生物样本的PDPN浓度变化，评估其在病理进程中的动态表达特征，为疾病分子机制研究及潜在治疗靶点开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aggrus ELISA Kit; Glycoprotein 36 KD ELISA Kit; Glycoprotein 36 ELISA Kit; gp 36 ELISA Kit; GP 38 ELISA Kit; GP 40 ELISA Kit; gp36 ELISA Kit; GP38 ELISA Kit; GP40 ELISA Kit; HT1A 1 ELISA Kit; HT1A1 ELISA Kit; hT1alpha 1 ELISA Kit; hT1alpha 2 ELISA Kit; hT1alpha1 ELISA Kit; hT1alpha2 ELISA Kit; Lung type I cell membrane associated glycoprotein ELISA Kit; Lung type I cell membrane associated glycoprotein isoform a ELISA Kit; Lung type I cell membrane associated glycoprotein T1A 2 ELISA Kit; OTS 8 ELISA Kit; OTS8 ELISA Kit; OTTHUMP00000009640 ELISA Kit; OTTHUMP00000044504 ELISA Kit; PA2.26 ELISA Kit; PA2.26 antigen ELISA Kit; Pdpn ELISA Kit; PDPN_HUMAN ELISA Kit; Podoplanin ELISA Kit; PSEC0003 ELISA Kit; PSEC0025 ELISA Kit; T1 alpha ELISA Kit; T1 ALPHA GENE ELISA Kit; T1-alpha ELISA Kit; T1A 2 ELISA Kit; T1A ELISA Kit; TI1A ELISA Kit; TIA 2 ELISA Kit; TIA2 ELISA Kit
缩写：PDPN
Uniprot No.：Q86YL7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:20 | Average % | 104 | ||||
| Range % | 99-108 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 85-91 | |||||
回收率：
| The recovery of human PDPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 105 | 103-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.6542.7952.7252.5378002.0222.2002.1111.9234001.2781.3691.3241.1362000.8010.8670.8340.6461000.4860.4920.4890.301500.3780.3630.3710.183250.2630.2820.2730.08500.1860.1890.188 | ||||||
货期：3-5 working days
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