首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠前蛋白转化酶枯草溶菌素9(PCSK9)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCSK9含量。PCSK9是一种重要靶点。其在调节肝脏表面低密度脂蛋白受体水平方面发挥关键作用。该靶点的异常会影响胆固醇代谢。相关研究聚焦于抑制PCSK9活性，以提升低密度脂蛋白受体数量，促进胆固醇清除，为降脂治疗提供新策略。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，该产品可广泛应用于心血管疾病机制研究、代谢综合征动物模型分析、肝脏功能相关实验以及药物开发过程中PCSK9调控效果的评估，为科研人员探究脂质代谢异常、动脉粥样硬化等领域的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pcsk9 ELISA Kit; Narc1 ELISA Kit; Proprotein convertase subtilisin/kexin type 9 ELISA Kit; EC 3.4.21.- ELISA Kit; Neural apoptosis-regulated convertase 1 ELISA Kit; NARC-1 ELISA Kit; Proprotein convertase 9 ELISA Kit; PC9 ELISA Kit; Subtilisin/kexin-like protease PC9 ELISA Kit
缩写：PCSK9
Uniprot No.：P59996
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：46.88 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 82-98 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 82-95 | |||||
回收率：
| The recovery of rat PCSK9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 60000.0880.0870.088 30000.1850.1880.187 15000.3580.3690.364 7500.6240.6300.627 3751.1301.1111.121 187.51.5161.5001.508 93.751.8101.7121.761 02.1692.0642.117 | ||||||
货期：3-5 working days
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