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产品描述：人前蛋白转化酶枯草杆菌蛋白酶kexin 9(PCSK9)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCSK9含量。PCSK9是重要的血脂调节靶点。其背景在于它能与低密度脂蛋白受体结合使其降解，从而影响胆固醇代谢。研究机制主要围绕抑制PCSK9活性，以减少低密度脂蛋白受体降解，增加其对血液中胆固醇清除，为降脂治疗提供新方向。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于基础研究中探索PCSK9在脂质代谢调控中的作用机制、评估药物或基因干预对PCSK9表达的影响，或用于心血管疾病模型中的生物标志物分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Convertase subtilisin/kexin type 9 preproprotein ELISA Kit; FH3 ELISA Kit; HCHOLA3 ELISA Kit; Hypercholesterolemia autosomal dominant 3 ELISA Kit; LDLCQ1 ELISA Kit; NARC 1 ELISA Kit; NARC-1 ELISA Kit; NARC1 ELISA Kit; Neural apoptosis regulated convertase 1 ELISA Kit; Neural apoptosis-regulated convertase 1 ELISA Kit; PC 9 ELISA Kit; PC9 ELISA Kit; PCSK 9 ELISA Kit; PCSK9 ELISA Kit; PCSK9_HUMAN ELISA Kit; Proprotein convertase 9 ELISA Kit; Proprotein convertase PC9 ELISA Kit; Proprotein convertase subtilisin/kexin type 9 ELISA Kit; PSEC0052 ELISA Kit; Subtilisin/kexin like protease PC9 ELISA Kit; Subtilisin/kexin-like protease PC9 ELISA Kit
缩写：PCSK9
Uniprot No.：Q8NBP7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:200 | Average % | 101 | ||||
| Range % | 94-106 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of human PCSK9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 101 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 300.0900.0860.088 150.2280.2410.235 7.50.3510.3720.362 3.750.5730.5980.586 1.80.9270.9360.932 0.91.6071.6321.620 0.451.9071.8761.892 02.6552.6152.635 | ||||||
货期：3-5 working days
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