首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠前白蛋白(PA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PA含量。试剂盒检测范围为375 ng/mL-6000 ng/mL，在肝损伤模型、营养干预研究及相关疾病机制探索中具有重要科研价值；可用于评估肝细胞功能、监测培养体系中蛋白质分泌动态，或分析组织样本病理生理变化；满足基础研究中对PA定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ttr ELISA Kit; Tt ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit; TBPA ELISA Kit
缩写：PA
Uniprot No.：P02767
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：375 ng/mL-6000 ng/mL
灵敏度：375 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 95-103 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of rat PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-103 | ||||
| EDTA plasma (n=4) | 89 | 82-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 60000.1520.1630.158 30000.2880.3000.294 15000.5610.5680.565 7500.9030.9250.914 3751.4771.4341.456 02.6622.5912.627 | ||||||
货期：3-5 working days
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