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产品描述：人蛋白质二硫键异构酶(PDI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的P4HB含量。P4HB是一种重要的靶点。它在细胞内参与蛋白质的折叠和稳定，尤其在胶原蛋白合成过程中发挥关键作用。相关研究聚焦于其作用机制，探索其与疾病发生发展的联系，期望通过调控P4HB来开发针对相关疾病的治疗方法。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于体外研究PDI在细胞应激调控、蛋白质稳态失衡相关疾病机制中的表达变化，或用于筛选调节PDI活性的化合物，为分子生物学、药理学及病理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cellular thyroid hormone binding protein ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Collagen prolyl 4 hydroxylase beta ELISA Kit; Disulphide Isomerase ELISA Kit; DSI ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERBA2L ELISA Kit; ERp59 ELISA Kit; GIT ELISA Kit; Gltathione insulin transhydrogenase ELISA Kit; Glutathione insulin transhydrogenase ELISA Kit; P4HB ELISA Kit; P4Hbeta ELISA Kit; p55 ELISA Kit; PDI ELISA Kit; PDIA1 ELISA Kit; PDIA1_HUMAN ELISA Kit; PDIR ELISA Kit; PHDB ELISA Kit; PO4DB ELISA Kit; PO4HB ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit ELISA Kit; PROHB ELISA Kit; Prolyl 4 hydroxylase beta polypeptide ELISA Kit; Prolyl 4 hydroxylase beta subunit ELISA Kit; Prolyl 4 hydroxylase subunit beta ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; Protein disulfide isomerase associated 1 ELISA Kit; Protein disulfide isomerase, family A, member 1 ELISA Kit; Protein disulfide isomerase/oxidoreductase ELISA Kit; Protein disulfide-isomerase ELISA Kit; Protocollagen hydroxylase ELISA Kit; Thbp ELISA Kit; Thyroid hormone binding protein p55 ELISA Kit; Thyroid hormone binding protein p55 cellular ELISA Kit; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like ELISA Kit
缩写：P4HB
Uniprot No.：P07237
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human PDI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7242.5872.6562.518102.1252.0082.0671.92951.3681.3971.3831.2452.50.8420.8310.8370.6991.250.5260.5380.5320.3940.6250.3450.3340.3400.2020.3120.2620.2540.2580.12000.1390.1370.138 | ||||||
货期：3-5 working days
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