首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠催产素(OT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆.样本中的OT含量。大鼠催产素（OT）是一种由九个氨基酸组成的神经肽，主要在下丘脑室旁核和视上核合成，通过激活G蛋白偶联受体（GPCR）发挥功能，调节分娩、哺乳、社交行为等。其释放的时空动态及分子机制尚不明晰，近年来，北京大学生命科学学院李毓龙实验室开发了新型催产素荧光探针GRABOT1.0，深入探究了催产素释放的分子机制。试剂盒检测范围为7.5 pg/mL-600 pg/mL，适用于神经内分泌研究、行为学实验及压力反应机制等科研领域。科研人员可通过该产品深入探究OT在焦虑调控、分娩启动或社会互动中的分子机制，为神经生物学和内分泌学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma.
检测范围：7.5 pg/mL-600 pg/mL
灵敏度：9.375 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 92 | 89-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 6000.3670.3870.377 2250.5120.5430.528 112.50.6680.6440.656 37.50.9560.9290.943 7.51.6981.6861.692 02.0852.0992.092 | ||||||
货期：3-5 working days
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