首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人催产素(OT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OT含量。人催产素（OT）是一种哺乳动物激素，由9个氨基酸构成，主要在大脑下视丘分泌，参与分娩、泌乳和母婴关系等。其机制研究显示，OT通过调节神经递质和受体基因，影响信任、合作和社会认知等行为，具有临床应用潜力，如治疗自闭症和社交恐惧症。试剂盒检测范围为8 μIU/mL-400 μIU/mL，适用于基础科研中探索神经生物学机制、行为学关联性分析或母婴健康相关研究；该产品为神经科学、心理学及内分泌学领域的研究者提供可靠工具，用于定量分析生物样本中OT的动态变化，支持探索其在生理调控及疾病模型中的潜在作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：OT
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：8 μIU/mL-400 μIU/mL
灵敏度：10 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2AverageCorrected 4002.4702.4982.4842.440 1601.5301.4961.5131.469 600.6490.6520.6510.607 200.2990.3130.3060.262 80.1470.1440.1460.102 00.0450.0430.044 | ||||||
货期：3-5 working days
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