首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨织素(Ostn)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OSTN含量。人骨织素（Ostn）是一种由骨骼细胞分泌的糖蛋白，参与骨代谢调控和能量稳态维持。其研究机制涉及调节细胞粘附、迁移、增殖和分化等生物学功能，通过整合素受体激活细胞内信号通路，影响细胞生长和行为。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研场景，如探索骨骼疾病机制、评估骨代谢相关生物标志物或研究能量代谢调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OSTN ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
缩写：OSTN
Uniprot No.：P61366
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ostn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human Ostn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 97 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5522.6992.6262.510102.2542.3732.3142.19851.8041.9281.8661.7502.50.8890.9260.9080.7921.250.5170.4980.5080.3920.6250.3200.3040.3120.1960.3120.2160.2020.2090.09300.1180.1140.116 | ||||||
货期：3-5 working days
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