首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人破骨细胞相关免疫球蛋白样受体(OSCAR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OSCAR含量。人破骨细胞相关免疫球蛋白样受体OSCAR是破骨细胞形成过程中的关键分子，能促进破骨细胞存活和分化，参与骨重建和免疫调节。其识别胶原蛋白，通过免疫球蛋白样受体协同刺激和承接分子作用，调节破骨细胞生成和免疫细胞功能。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于细胞生物学研究、疾病动物模型骨代谢评估、骨相关病理机制探索及药物开发过程中靶点活性监测等科研场景，为骨代谢调控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OSCAR ELISA Kit; Osteoclast-associated immunoglobulin-like receptor ELISA Kit; Osteoclast-associated receptor ELISA Kit; hOSCAR ELISA Kit; Polymeric immunoglobulin receptor 3 ELISA Kit; PIgR-3 ELISA Kit; PIgR3 ELISA Kit; Poly-Ig receptor 3 ELISA Kit
缩写：OSCAR
Uniprot No.：Q8IYS5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 85-91 | |||||
回收率：
| The recovery of human OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.6042.7882.6962.5352002.1682.0332.1011.9401001.4231.5211.4721.311500.8780.8600.8690.708250.6360.6230.6300.46912.50.4140.4540.4340.2736.250.2330.2460.2400.07900.1560.1660.161 | ||||||
货期：3-5 working days
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