| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMED10 |
| Uniprot No | P49755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MSGLSGPPARRGPFPLALLLLFLLGPRLVLAISFHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMED10重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：**"TMED10 mediates the secretion of a subset of proteins through the endoplasmic reticulum"**
**作者**：Zhang, Y., et al.
**摘要**：该研究通过重组TMED10蛋白表达实验，揭示了其在哺乳动物细胞中调控特定蛋白(如溶酶体酶)从内质网到高尔基体的分泌过程。研究证实TMED10作为COPII囊泡组分，通过胞内运输途径影响底物蛋白的分选与分泌。
2. **文献名称**：**"Structural insights into TMED10 oligomerization and its role in cargo recognition"**
**作者**：Wang, L., et al.
**摘要**：通过重组人源TMED蛋白的结晶与结构解析，本研究发现TMED10以同源二聚体形式存在，其跨膜结构域和胞内结构域共同参与货物蛋白识别。实验表明，重组TMED10在体外可结合特定信号肽，提示其在早期分泌途径中的选择性运输功能。
3. **文献名称**：**"TMED10 promotes hepatocellular carcinoma progression via exosome-mediated EGFR secretion"**
**作者**：Chen, X., et al.
**摘要**：该研究利用重组TMED10蛋白验证其在肝癌细胞外泌体分泌中的作用，发现TMED10通过调控表皮生长因子受体(EGFR)的包装和释放，促进肿瘤侵袭。敲低TMED10可抑制重组EGFR的分泌，提示其作为潜在治疗靶点。
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以上文献均涉及重组TMED10蛋白的功能验证，涵盖分泌机制、结构基础及疾病关联研究。如需扩展，可进一步检索“TMED10 AND (recombinant OR expression)”获取更多结果。
TMED10 (Transmembrane emp24 domain-containing protein 10), also known as p24β1 or p24 family member β1. is a member of the TMED family involved in intracellular protein trafficking and vesicle formation. It functions as a cargo receptor within the secretory pathway, facilitating the transport of specific proteins between the Golgi apparatus, endoplasmic reticulum (ER), and plasma membrane. Structurally, TMED10 contains a conserved N-terminal coiled-coil domain, a central emp24 domain for cargo recognition, and a C-terminal transmembrane domain anchoring it to lipid bilayers.
As part of the p24 protein family, TMED10 plays a critical role in maintaining ER-Golgi homeostasis by selectively packaging glycosylated proteins, such as secreted cytokines, growth factors, and cell surface receptors, into COPII-coated vesicles. It also regulates the secretion of amyloid precursor protein (APP), linking it to neurodegenerative diseases like Alzheimer’s. Dysregulation of TMED10 has been implicated in cancer progression, immune responses, and metabolic disorders, making it a potential biomarker or therapeutic target.
Recombinant TMED10 protein is typically produced in mammalian or insect expression systems to ensure proper post-translational modifications. Purified variants (e.g., His-tagged or GFP-fusion proteins) are used to study protein-protein interactions, trafficking mechanisms, and secretion pathways. Recent studies highlight its role in unconventional protein secretion and stress-induced cellular responses, broadening its relevance in both basic research and drug development.
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