首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α1酸性糖蛋白(α1-AGP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液、组织匀浆样本中的ORM1含量。ORM1即阿片受体μ样1，是一种重要的药物靶点。研究发现它参与痛觉调节、情绪调控等生理过程。其机制主要在于与内源性配体结合后，通过一系列信号转导影响细胞功能，在镇痛、精神疾病治疗等方面有潜在研究价值。试剂盒检测范围为3.125 ng/mL-800 ng/mL，具备高灵敏度和特异性，适用于炎症相关机制研究、药物干预效果评价或疾病动物模型中的生物标志物分析等科研场景，实验操作流程标准化，配套试剂完整，可满足实验室常规检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
缩写：ORM1
Uniprot No.：P02764
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine, cell culture supernates, tissue homogenates
检测范围：3.125 ng/mL-800 ng/mL
灵敏度：3.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 95 | |||||
| Range % | 85-105 | ||||||
| 1:2000 | Average % | 89 | |||||
| Range % | 81-98 | ||||||
| 1:4000 | Average % | 90 | |||||
| Range % | 82-97 | ||||||
| 1:8000 | Average % | 95 | |||||
| Range % | 83-98 | ||||||
回收率：
| The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-102 | |||||
| EDTA plasma (n=4) | 95 | 90-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average8000.2130.2170.2152000.4590.4530.456500.8510.8590.85512.51.2261.2461.2363.1251.7271.7401.73401.9351.9581.947 | |||||||
货期：3-5 working days
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