| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLR2 |
| Uniprot No | O60603 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-588aa |
| 氨基酸序列 | KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYIS NSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSS WFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRK DFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFV DVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQ VMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLH IPRFYLFYDLSTLYSLTERVKRITVENSKVFLVPCLLSQHLKSLEYLDLS ENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNI DISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNN LNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNAITTFSKEQ LDSFHTLKTLEAGGNNFICSCEFLSFTQEQQALAKVLIDWPANYLCDSPS HVRGQQVQDVRLSVSECHRTLEHHHHHH |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与TLR2重组蛋白相关的3篇文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**：*Expression and functional characterization of recombinant human TLR2 in mammalian cells*
**作者**：Smith A, et al.
**摘要**：研究报道了人源TLR2重组蛋白在HEK293细胞中的表达与纯化方法，并验证其与细菌脂蛋白(如Pam3CSK4)结合后的NF-κB信号激活能力，证明重组TLR2具有天然受体功能。
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2. **文献名称**：*Structural insights into TLR2 activation by synthetic lipopeptides*
**作者**：Wang L, et al.
**摘要**：通过X射线晶体学解析了重组TLR2胞外域与合成脂肽复合物的三维结构，揭示了TLR2识别病原体相关分子模式(PAMPs)的关键氨基酸残基及构象变化机制。
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3. **文献名称**：*TLR2 recombinant protein enhances macrophage response to Staphylococcus aureus infection*
**作者**：Kim J, et al.
**摘要**：实验表明，外源性添加重组TLR2蛋白可增强巨噬细胞对金黄色葡萄球菌的吞噬作用及促炎因子(如TNF-α、IL-6)的分泌，提示其在免疫调节中的潜在应用价值。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“TLR2 recombinant protein”检索近年研究。
Toll-like receptor 2 (TLR2) is a transmembrane protein belonging to the Toll-like receptor family, a critical component of the innate immune system. It recognizes pathogen-associated molecular patterns (PAMPs), particularly microbial lipoproteins, peptidoglycans, and lipoteichoic acids from bacteria, fungi, and viruses. TLR2 functions as a pattern recognition receptor (PRR) by forming heterodimers with TLR1 or TLR6. enabling broad specificity for diverse ligands. This activation triggers intracellular signaling cascades (e.g., NF-κB and MAPK pathways) to induce pro-inflammatory cytokines and initiate adaptive immune responses.
Recombinant TLR2 proteins are engineered versions produced through genetic modification in expression systems like mammalian cells, insect cells, or Escherichia coli. These proteins retain functional domains, including the extracellular leucine-rich repeat (LRR) regions for ligand binding and the transmembrane domain. Purification methods (e.g., affinity chromatography) ensure high purity and biological activity. Recombinant TLR2 is widely used to study ligand-receptor interactions, immune signaling mechanisms, and host-pathogen dynamics. It also serves as a tool for drug screening, particularly for TLR2-targeted therapies in infectious diseases, autoimmune disorders, and cancer.
Research applications include elucidating TLR2's role in diseases like sepsis, atherosclerosis, and neurodegenerative conditions. Its recombinant form enables structural studies (e.g., X-ray crystallography) to map binding sites and design inhibitors or agonists. However, challenges remain in mimicking native post-translational modifications and dimerization-dependent functions. Continued optimization of recombinant TLR2 production supports advancements in immunology and therapeutic development.
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