首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠阿立新A(Orexin A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Orexin A含量。Orexin A 是一种神经肽，在调节觉醒、食欲等生理过程中发挥重要作用。研究发现其与睡眠-觉醒周期、能量代谢等密切相关，作用机制主要是通过与特定受体结合，激活相关信号通路，影响神经元活动和神经递质释放。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经科学、代谢研究等领域，可帮助研究者通过血清或血浆样本分析Orexin A在应激反应、昼夜节律实验或代谢综合征动物模型中的表达特征，为探索神经肽调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hcrt ELISA Kit; Ox ELISA Kit; Ppox ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
缩写：Orexin A
Uniprot No.：O55232
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 84-107 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-103 | |||||
回收率：
| The recovery of rat orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 88 | 83-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1082.0762.0921.9785001.7741.7851.7801.6662501.4421.4681.4551.3411251.1401.1491.1451.03162.50.7440.7630.7540.64031.20.5250.5340.5300.41615.60.2910.2820.2870.17300.1130.1140.114 | ||||||
货期：3-5 working days
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