首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经肽Nesfatin-1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Nesfatin-1含量。Nesfatin-1是一个值得关注的靶点。它在体内广泛分布，与能量代谢、进食调节等生理过程密切相关。目前研究聚焦于其作用机制，如通过与特定受体结合，影响神经信号传导，调节代谢相关激素分泌，为肥胖、代谢疾病治疗带来新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于代谢疾病模型研究、肥胖相关神经内分泌机制探索，或通过检测脑组织、外周血等样本动态观察Nesfatin-1在应激状态下的表达变化，为研究能量代谢调控、神经肽信号通路提供可靠工具，满足基础科研中对低丰度靶标的高效检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Nesfatin-1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 80-105 | |||||
| 1:10 | Average % | 99 | ||||
| Range % | 91-111 | |||||
| 1:20 | Average % | 104 | ||||
| Range % | 92-112 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6172.5572.5872.46310002.2052.3562.2812.1575001.6361.7671.7021.5782501.0431.0581.0510.9271250.6090.6420.6260.50262.50.3520.3460.3490.22531.250.2260.2430.2350.11100.1220.1250.124 | ||||||
货期：3-5 working days
×
