| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BBC1; L13; rpl13;;RPL13 |
| WB Predicted band size | Calculated MW: 24 kDa ; Observed MW: 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human RPL13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RPL13抗体的3篇参考文献示例(基于公开研究主题概括，非真实文献)：
1. **标题**：*RPL13 as a regulator of inflammatory cytokine production in macrophages*
**作者**：Smith A, et al.
**摘要**：研究利用RPL13抗体通过Western blot和免疫沉淀技术，发现RPL13在巨噬细胞中通过核糖体非依赖途径调控TNF-α和IL-6的表达，揭示其在炎症反应中的新功能。
2. **标题**：*Ribosomal protein L13 expression correlates with tumor progression in colorectal cancer*
**作者**：Zhang L, et al.
**摘要**：通过免疫组化(使用RPL13抗体)分析结直肠癌组织样本，发现RPL13高表达与患者生存率降低及转移相关，提示其作为癌症预后标志物的潜力。
3. **标题**：*Specificity validation of commercial RPL13 antibodies for translational research*
**作者**：Johnson R, et al.
**摘要**：评估了多种市售RPL13抗体的特异性，通过siRNA敲低实验和质谱验证，确认某品牌抗体在细胞系和小鼠模型中的可靠性，为相关研究提供抗体选择依据。
4. **标题**：*RPL13-mediated translational control in obesity-associated metabolic syndrome*
**作者**：Lee H, et al.
**摘要**：利用RPL13抗体进行免疫荧光染色，发现肥胖模型中RPL13在肝脏细胞的亚定位变化影响脂代谢相关mRNA的翻译效率，揭示其在代谢疾病中的作用机制。
(注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。)
The RPL13 antibody is a key tool for studying the ribosomal protein L13. a component of the 60S ribosomal subunit involved in protein synthesis. Beyond its canonical role, RPL13 has gained attention for its non-ribosomal functions, particularly in immune and inflammatory responses. For example, RPL13 is a critical member of the GAIT (Gamma-Activated Inhibitor of Translation) complex, which binds to specific mRNA motifs to suppress translation of pro-inflammatory genes during interferon-γ signaling. This regulatory mechanism helps resolve inflammation and maintain cellular homeostasis.
RPL13 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to evaluate RPL13 expression, localization, and post-translational modifications. Dysregulation of RPL13 has been linked to diseases such as cancer, autoimmune disorders, and chronic inflammation, making these antibodies valuable in both basic and translational research. For instance, altered RPL13 levels are observed in certain cancers, suggesting its potential role in tumor progression or suppression.
Researchers also utilize RPL13 antibodies to explore ribosome stress responses, as ribosomal proteins like RPL13 can be released into the cytoplasm under stress, triggering p53-dependent cell cycle arrest or apoptosis. Specificity validation (e.g., siRNA knockdown or knockout controls) is essential, as commercial antibodies may vary in cross-reactivity. Overall, RPL13 antibodies bridge studies on ribosome biology, disease mechanisms, and therapeutic target discovery.
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