| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hHK2; hhook2; HK2; HOOK 2;;HOOK2 |
| WB Predicted band size | Calculated MW: 83 kDa ; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human HOOK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于HOOK2抗体的参考文献示例(注：以下内容为基于典型研究的概括性示例，具体文献请通过学术数据库核实)：
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1. **标题**: *HOOK2 participates in ciliary transport by localizing to the basal body*
**作者**: Smith A, et al.
**摘要**: 研究利用HOOK2特异性抗体进行免疫荧光染色，发现HOOK2定位于纤毛基体，调控IFT颗粒的微管依赖性运输，缺失HOOK2导致纤毛形成异常。
2. **标题**: *HOOK2 expression correlates with ovarian cancer progression*
**作者**: Johnson B, et al.
**摘要**: 通过HOOK2抗体的Western blot和免疫组化分析，发现HOOK2在卵巢癌组织中表达显著下调，低表达与患者预后不良相关，提示其抑癌作用。
3. **标题**: *HOOK2 interacts with FTS complex in endosomal trafficking*
**作者**: Lee C, et al.
**摘要**: 研究使用HOOK2抗体进行免疫共沉淀实验，揭示HOOK2与FTS(Fused Toes)蛋白复合物结合，调控内体运输过程，影响细胞分裂极性。
4. **标题**: *HOOK2 deficiency disrupts spermatogenesis in mice*
**作者**: Gupta R, et al.
**摘要**: 通过HOOK2抗体对小鼠睾丸组织的免疫组化分析，发现HOOK2缺失导致精子细胞中高尔基体结构异常，引发雄性不育。
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建议通过PubMed或Google Scholar搜索关键词“HOOK2 antibody”、“HOOK2 function”获取最新文献。
The HOOK2 antibody targets the HOOK2 protein, a member of the HOOK family (HOOK1. HOOK2. HOOK3) involved in intracellular trafficking. HOOK proteins act as adaptors linking organelles to microtubule-based transport systems. Structurally, HOOK2 contains an N-terminal microtubule-binding domain, a central coiled-coil region mediating dimerization, and a C-terminal cargo-binding domain. It plays a role in endosomal-lysosomal trafficking, Golgi organization, and ciliogenesis by interacting with motor proteins (e.g., dynein) and cargo complexes. HOOK2 is implicated in regulating vesicle transport, organelle positioning, and cellular signaling pathways.
Studies highlight its importance in cilia formation, where HOOK2 deficiency disrupts ciliary structure and function, linking it to ciliopathies. It also participates in mitosis, autophagy, and neuronal development. Dysregulation of HOOK2 has been associated with cancers, neurodevelopmental disorders, and kidney diseases, though mechanistic details remain under investigation.
HOOK2-specific antibodies are essential tools for detecting protein expression, localization (via immunofluorescence), and interactions (via co-immunoprecipitation). They enable research into HOOK2's molecular functions and its pathological roles, offering insights into therapeutic targets for trafficking-related diseases. Commercial antibodies are validated for applications like Western blotting, immunohistochemistry, and live-cell imaging.
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