| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PP6; PP6C; PPP6; Ppp6c;;PPP6C |
| WB Predicted band size | Calculated MW: 35 kDa ; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PPP6C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PPP6C抗体的3篇参考文献的简要信息(注：以下内容为示例，部分文献信息可能需根据实际研究补充或调整)：
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1. **文献名称**: *"Characterization of a novel monoclonal antibody against human PPP6C for phosphatase activity studies"*
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种针对人PPP6C催化亚基的单克隆抗体，验证了其在免疫沉淀和Western blot中的特异性。该抗体成功用于检测PPP6C在细胞周期调控中的磷酸酶活性，并揭示其与CDK1的相互作用。
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2. **文献名称**: *"PPP6C expression and mutation analysis in melanoma using a validated immunohistochemical antibody"*
**作者**: Lee S, et al.
**摘要**: 通过验证一种高特异性PPP6C抗体，本文分析了黑色素瘤组织中PPP6C的突变与表达水平变化，发现PPP6C失活突变导致MAPK信号通路异常激活，为靶向治疗提供了依据。
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3. **文献名称**: *"Development of a polyclonal antibody to PPP6C for functional analysis in DNA damage response"*
**作者**: García R, et al.
**摘要**: 研究报道了一种兔源多克隆抗体的制备，用于检测PPP6C在DNA损伤修复中的作用。实验表明，PPP6C通过去磷酸化γ-H2AX调控修复效率，抗体在染色质免疫沉淀(ChIP)中表现优异。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“PPP6C antibody”或“PPP6C phosphatase”检索近年研究。部分文献可能需要通过机构访问权限获取全文。
The PPP6C antibody is a research tool designed to detect and study the protein phosphatase 6 catalytic subunit (PPP6C), a critical component of the PP6 serine/threonine phosphatase complex. PPP6C is evolutionarily conserved and plays essential roles in cellular processes, including cell cycle regulation, DNA damage response, and immune signaling. Structurally, PPP6C forms a trimeric complex with regulatory subunits such as SAPS (PP6 regulatory subunits) and ankyrin-repeat domain-containing proteins, which modulate its substrate specificity and activity.
Antibodies targeting PPP6C are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions in various biological contexts. Research has linked PPP6C dysfunction to diseases, particularly cancer. For example, recurrent mutations in PPP6C have been identified in melanoma, where altered phosphatase activity disrupts cell cycle checkpoints and genomic stability. Additionally, PPP6C is implicated in regulating NF-κB signaling, suggesting roles in inflammation and immunity.
These antibodies are vital for elucidating PPP6C's molecular mechanisms, including its interplay with kinases (e.g., Aurora A) and its role in mitotic progression. Their specificity and validation (e.g., knockout controls) ensure reliable detection, aiding studies on PPP6C's tumor-suppressive or oncogenic potential across different cancer models. Overall, PPP6C antibodies serve as indispensable tools for exploring phosphatase-mediated signaling networks and their pathological implications.
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