| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DMB; RING7;;HLA DMB |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human HLA DMB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于HLADMB抗体的3篇代表性文献(内容为示例，建议通过学术数据库核实具体信息)：
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1. **文献名称**：*"HLA-DM acts as a molecular chaperone and rescues empty HLA-DR molecules in lysosomal compartments"*
**作者**：Kropshofer, H., et al.
**摘要**：研究HLA-DM(含HLADMB亚基)在MHC II类分子抗原呈递中的作用，发现其通过结合空载的HLA-DR分子促进抗原肽加载。实验中利用HLADMB抗体阻断功能，证实其对溶酶体中抗原加工的关键调控。
2. **文献名称**：*"Genetic polymorphism of HLA-DMB and susceptibility to rheumatoid arthritis"*
**作者**：Morris, D.L., et al.
**摘要**：探讨HLADMB基因多态性与类风湿性关节炎(RA)的关联，通过抗体检测患者外周血单核细胞中HLADMB蛋白表达水平，发现特定等位基因可能通过调控表达量影响RA易感性。
3. **文献名称**：*"Structural and functional characterization of HLA-DM mutants reveals key mechanisms of antigen peptide exchange"*
**作者**：Denzin, L.K., et al.
**摘要**：利用HLADMB特异性抗体进行免疫沉淀和结构分析，揭示HLA-DM通过构象变化促进MHC II类分子的抗原肽置换，为自身免疫疾病治疗提供潜在靶点。
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**备注**：上述文献为简化示例，实际研究中HLADMB常作为HLA-DM复合体的一部分被研究，建议结合关键词“HLA-DM”或“HLA class II”扩展检索。
The HLA-DMB antibody is a crucial tool in studying the human leukocyte antigen (HLA) class II system, specifically targeting the HLA-DM β-chain encoded by the HLA-DMB gene. HLA-DM is a non-classical MHC class II heterodimer (composed of HLA-DMA and HLA-DMB subunits) that plays a pivotal role in antigen presentation. Unlike classical HLA class II molecules, HLA-DM does not present antigens directly but acts as a chaperone, facilitating the exchange of invariant chain-derived peptides (CLIP) with exogenous antigenic peptides in endosomal compartments. This process ensures proper loading of antigenic peptides onto HLA class II molecules for recognition by CD4+ T cells, a critical step in adaptive immunity.
HLA-DMB antibodies are widely used in immunology research to investigate HLA-DM expression, localization, and function in antigen-processing pathways. They help elucidate mechanisms underlying autoimmune diseases, infections, and immune deficiencies linked to HLA-DM dysregulation. For example, altered HLA-DM activity has been implicated in conditions like rheumatoid arthritis, type 1 diabetes, and certain viral infections. These antibodies are employed in techniques such as flow cytometry, Western blotting, and immunohistochemistry to assess protein expression in cell lines, primary immune cells, or tissue samples. Commercial HLA-DMB antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of the β-chain, and validated for specificity and sensitivity in diverse experimental models.
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