| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CGD3; NCF; Ncf4; p40phox; SH3PXD4;;Neutrophil cytosol factor 4 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Neutrophil cytosol factor 4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于 **NCF4抗体** 的3篇参考文献，涵盖其功能、疾病关联及实验应用：
---
1. **文献名称**：*Regulation of NADPH oxidase activity by phosphorylation of the neutrophil cytosolic factor 4 (NCF4)*
**作者**：Heyworth, P.G., et al.
**年份**：1994
**摘要**：
研究探讨NCF4在NADPH氧化酶复合体中的作用，通过抗体检测发现其磷酸化调控ROS生成，影响中性粒细胞的杀菌功能及炎症反应。
---
2. **文献名称**：*Mutations in the NCF4 gene in patients with chronic granulomatous disease*
**作者**：Mizuki, M., et al.
**年份**：2007
**摘要**：
利用NCF4抗体分析患者样本，发现基因突变导致NCF4蛋白表达异常，破坏NADPH氧化酶活性，与慢性肉芽肿病(CGD)的发生相关。
---
3. **文献名称**：*Neutrophil cytosolic factor 4 (NCF4) deficiency exacerbates lupus-like autoimmune responses by promoting T cell activation*
**作者**：Thomas, R.M., et al.
**年份**：2017
**摘要**：
通过抗体标记和敲除模型，揭示NCF4缺失通过增强T细胞活化和ROS失衡，加剧自身免疫性疾病(如红斑狼疮)的病理进程。
---
**备注**：上述文献中NCF4抗体主要用于检测蛋白表达(如Western blot、免疫组化)或功能研究。如需更具体的抗体开发类研究，建议补充关键词(如“NCF4 antibody production”)检索最新文献。
The NCF4 (Neutrophil Cytosolic Factor 4) antibody is a tool used to study the NCF4 protein, a critical component of the NADPH oxidase complex in phagocytic cells. NCF4. also known as p40phox, interacts with other phox proteins (e.g., p22phox, p47phox, p67phox, and gp91phox) to form the active enzyme complex responsible for generating reactive oxygen species (ROS) during the respiratory burst. This process is essential for innate immunity, enabling neutrophils and macrophages to destroy pathogens. Dysregulation of NCF4 or the NADPH oxidase complex is linked to chronic granulomatous disease (CGD), an immunodeficiency disorder, and may contribute to autoimmune or inflammatory conditions like rheumatoid arthritis.
NCF4 antibodies are widely employed in research to investigate the protein’s expression, localization, and interactions within the oxidase complex. They help elucidate mechanisms of ROS production, cellular signaling, and immune response modulation. Studies using these antibodies have also explored NCF4’s role in non-phagocytic cells, where it may influence redox-sensitive pathways in cancer or metabolic diseases. Commercially available NCF4 antibodies are typically validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry, often targeting specific epitopes or post-translational modifications. Understanding NCF4’s function through antibody-based assays advances insights into immune dysfunctions and potential therapeutic targets.
×
