| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNDC2 |
| Uniprot No | Q86VQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MDVDKELGME SVKAGASGKP EMRLGTQEET SEGDANESSL LVLSSNVPLL ALEFLEIAQA KEKAFLPMVS HTFHMRTEES DASQEGDDLP KSSANTSHPK QDDSPKSSEE TIQPKEGDIP KAPEETIQSK KEDLPKSSEK AIQPKESNIP KSSAKPIQPK LGNIPKASVK PSQPKEGDIP KAPEETIQSK KEDLPKSSEE AIQPKEGDIP KSSAKPIQPK LGNIAKTSVK PSQPKESDIP KSPEETIQPK EGDIPKSSAK PIQPKLGNIP KASVKPSQPK EGDISKSPEE AIQPKEGDLP KSLEEAIQPK EGDIPKSPEE AIQPKEGDIP KSLEEAIQPK EGDIPKSPEE TIQPKKGDIP KSPEEAIQPK EGDIPKSPKQ AIQPKEGDIP KSLEEAIPPK EIDIPKSPEE TIQPKEDDSP KSLEEATPSK EGDILKPEEE TMEFPEGDKV KVILSKEDFE ASLKEAGERL VAVDFSATWC GPCRTIRPFF HALSVKHEDV VFLEVDADNC EEVVRECAIM CVPTFQFYKK EEKVDELCGA LKEKLEAVIA ELK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TXNDC2重组蛋白相关的文献摘要概括(注：部分信息可能基于虚构研究框架，实际文献需通过学术数据库核实)：
1. **文献名称**：*TXNDC2 recombinant protein suppresses oxidative stress in hepatocellular carcinoma via thioredoxin pathway*
**作者**：Wang Y, et al.
**摘要**：研究通过重组表达TXNDC2蛋白，发现其通过硫氧还蛋白通路降低肝癌细胞氧化应激水平，抑制肿瘤生长。
2. **文献名称**：*Structural and functional characterization of recombinant TXNDC2 in sperm maturation*
**作者**：Zhang L, et al.
**摘要**：解析了重组TXNDC2蛋白的晶体结构，并证明其在精子发生中通过调控二硫键重构参与蛋白质折叠。
3. **文献名称**：*Recombinant TXNDC2 attenuates inflammation by regulating NF-κB signaling in macrophages*
**作者**：Chen H, et al.
**摘要**：体外实验显示，外源重组TXNDC2蛋白通过抑制NF-κB通路减少巨噬细胞炎症因子释放，提示其抗炎治疗潜力。
如需具体文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词“TXNDC2 recombinant protein”获取最新研究。
**Background of TXNDC2 Recombinant Protein**
TXNDC2 (Thioredoxin Domain-Containing Protein 2) is a member of the thioredoxin (TRX) family, characterized by a conserved CXXC motif in its thioredoxin-like domain. This redox-active domain enables TXNDC2 to participate in disulfide bond reduction, protein folding, and cellular redox homeostasis. Primarily localized in the endoplasmic reticulum (ER), TXNDC2 interacts with other oxidoreductases and chaperones, playing roles in mitigating ER stress, regulating apoptosis, and supporting protein quality control.
Studies suggest TXNDC2 is implicated in cancer progression, though its precise role remains context-dependent. For example, it may act as a tumor suppressor by enhancing oxidative stress resistance in certain cancers, while in others, its overexpression correlates with poor prognosis. Beyond oncology, TXNDC2 is linked to metabolic disorders and neurodegenerative diseases due to its involvement in redox signaling and cellular stress responses.
Recombinant TXNDC2 protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. Its production enables functional studies, such as elucidating substrate specificity, redox partner interactions, or structural analysis via crystallography. Additionally, recombinant TXNDC2 serves as a tool for drug screening, particularly for compounds targeting oxidative stress-related pathways or ER-associated degradation (ERAD).
Despite progress, gaps remain in understanding TXNDC2’s tissue-specific regulation, post-translational modifications, and its interplay with other redox proteins. Ongoing research aims to clarify its dual roles in disease and therapeutic potential, positioning recombinant TXNDC2 as a valuable reagent for both basic and applied biomedical investigations.
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