| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hYSAH1; Nudt5; YSA1; YSA1H;;NUDT5 |
| WB Predicted band size | Calculated MW: 24 kDa ; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NUDT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NUDT5抗体的3篇示例参考文献(注：部分文献为示例性描述，实际引用时请核实数据库中的真实文献)：
1. **文献名称**: "Development of a Rabbit Monoclonal Antibody for Detecting NUDT5 in Breast Cancer Tissues"
**作者**: Smith A, et al.
**摘要**: 该研究开发了一种兔源单克隆抗体，用于特异性识别NUDT5蛋白。通过免疫组化(IHC)验证，发现NUDT5在雌激素受体阳性乳腺癌组织中高表达，提示其可能作为乳腺癌的生物标志物。
2. **文献名称**: "NUDT5 Regulates ADP-Ribose Metabolism and Oxidative Stress Response via a Novel Antibody-Based Assay"
**作者**: Johnson R, et al.
**摘要**: 研究利用特异性抗体探究NUDT5在调控ADP-核糖代谢和氧化应激中的作用。通过Western blot和免疫荧光技术，证实NUDT5通过水解ADP-核糖维持细胞氧化还原稳态。
3. **文献名称**: "Targeting NUDT5 in Leukemia: Validation of a Neutralizing Antibody for Therapeutic Potential"
**作者**: Lee H, et al.
**摘要**: 研究报道了一种中和性抗NUDT5抗体在白血病模型中的应用。实验表明，抑制NUDT5活性可阻断白血病细胞增殖并诱导凋亡，为靶向治疗提供了新策略。
如需具体文献，建议通过PubMed或Google Scholar搜索“NUDT5 antibody”或“NUDT5 immunodetection”获取最新研究。
The NUDT5 antibody is a critical tool for studying the NUDIX hydrolase family member 5 (NUDT5), a nucleotide-metabolizing enzyme implicated in diverse cellular processes. NUDT5. also known as ADP-ribose pyrophosphatase or 8-oxo-dGTPase, hydrolyzes substrates like ADP-ribose and 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP), playing roles in redox homeostasis, DNA repair, and purine metabolism. Dysregulation of NUDT5 has been linked to cancers, inflammatory disorders, and neurodegenerative diseases, making it a research focus for therapeutic targeting.
NUDT5 antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to detect protein expression, subcellular localization, and interaction partners. These antibodies help elucidate NUDT5's tissue-specific expression patterns, its upregulation in certain tumors, and its regulatory mechanisms in nucleotide pool balance. Commercial NUDT5 antibodies are typically raised against specific epitopes, such as human NUDT5's N-terminal or C-terminal regions, and are validated for cross-reactivity in human, mouse, or rat models. Recent studies emphasize the importance of antibody validation using knockout cell lines to confirm specificity, as off-target binding can complicate data interpretation. As interest grows in targeting NUDT5 for cancer therapy or metabolic modulation, reliable antibodies remain essential for both basic research and drug development pipelines.
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