Human Nephrin ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E09886h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人肾病蛋白(nephrin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NPHS1含量。NPHS1编码足突蛋白，在维持肾小球滤过屏障功能中至关重要。其突变会引发先天性肾病综合征等疾病。研究机制围绕其在足细胞结构与功能维持上的作用，探究其如何保障滤过屏障正常，为相关肾病诊断和治疗提供靶点。试剂盒检测范围为1.56 ng/ml-100ng/ml，该产品适用于肾脏疾病机制研究、足细胞功能评估及药物干预模型中nephrin表达水平的检测，尤其适合探究蛋白尿相关病理过程中足细胞损伤的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CNF ELISA Kit; Nephrin ELISA Kit; Nephrosis 1 congenital Finnish type ELISA Kit; Nephrosis 1, congenital, Finnish type (nephrin) ELISA Kit; NPHN ELISA Kit; NPHN_HUMAN ELISA Kit; NPHS 1 ELISA Kit; Nphs1 ELISA Kit; Renal glomerulus specific cell adhesion receptor ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
缩写：
NPHS1
Uniprot No.：
O60500
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
1.56 ng/ml-100ng/ml
灵敏度：
0.39 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human nephrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	102
	Range %	92-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	88
	Range %	84-94
1:8	Average %	87
	Range %	82-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
100	2.566	2.465	2.516	2.370
50	1.859	1.758	1.809	1.663
25	1.048	1.027	1.038	0.892
12.5	0.625	0.614	0.620	0.474
6.25	0.402	0.412	0.407	0.261
3.12	0.267	0.278	0.273	0.127
1.56	0.222	0.226	0.224	0.078
0	0.146	0.145	0.146